GSM3130818: LPS_IC_DAPT_REP2_002; Mus musculus; RNA-Seq - SRA

SRX4037341: GSM3130818: LPS_IC_DAPT_REP2_002; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 14.2M spots, 2G bases, 879.6Mb downloads

Submitted by: NCBI (GEO)

Study: Effect of Notch Signaling Inhibitor on Gene Expression in Lipopolysaccharide-stimulated Macrophages in the Presence of Immune Complex

PRJNA454891 • SRP144516 • All experiments • All runs

show Abstracthide Abstract

Lipopolysaccharide (LPS)/immunue complex (IC) stimulated macrophages produce cytokine profiles that differe from LPS-stimulated inflammatory macropahges. Notch signaling is activated in LPS/IC-stimulated macrophages and inhibition of Notch signaling reduced IL-10 production. This study investigated the effect of gamma-secretase inhibitor (GSI) that suppresses Notch signaling pathway, on gene expression profiles in macrophages activated by LPS/IC. Overall design: Gamma secretase inhibitor (GSI)-treated bone marrow derived macrophage mRNA profiles were generated by deep sequencing using Illumina NextSeq 500. The results were compared between vehicle control (dimethylsulfoxide)-treated cells and GSI-treated cells.

Sample: LPS_IC_DAPT_REP2_002

SAMN09060745 • SRS3255056 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNA samples were extracted from LPS/IC-activated BMDMs in the presence of DMSO or GSI for 1 hr using an RNeasy Mini Kit (Qiagen, USA). RNA samples were assessed for quality using an Agilent 2100 Bioanalyzer (Agilent, USA) and quantified using a Qubit 3.0 fluorometer (Life Technologies, USA). RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM3130818

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 14.2M spots, 2G bases, 879.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR7109534	14,171,270	2G	879.6Mb	2018-06-22

ID:: 5513990

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