Instrument: Illumina HiSeq 2000
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: For ChIP, cultures were crosslinked with 1% (v/v) formaldehyde, quenched with glycine, and cells were lysed with beads in a FastPrep-24 (MP Biomedicals) cell homogeniser. Sonicated, clarified extracts were immunoprecipitated with anti-Htz1 antibodies before washing and DNA extraction, or were used directly to prepare input DNA. For RNA-seq, total RNA was purified using the Ribopure Purification Kit (Life Technologies) and contaminating genomic DNA was removed by DNaseI digestion. Illumina TruSeq ChIP Sample Prep Kit and Illumina TruSeq stranded mRNA sample preparation kits were used for library preparation.