GSM3319296: DGRP-426_Control; Drosophila melanogaster; RNA-Seq - SRA

SRX4510290: GSM3319296: DGRP-426_Control; Drosophila melanogaster; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 22.1M spots, 2.2G bases, 1.3Gb downloads

Submitted by: NCBI (GEO)

Study: RNA-sequencing of the female adult fly guts from 38 DGRP lines with and without oral infection with Pseudomonas entomophila

PRJNA484700 • SRP156430 • All experiments • All runs

show Abstracthide Abstract

In order to gain insights into gut immunocompetence and its variation in a population, we performed RNA-sequencing of whole guts of 38 Drosophila Genetic Reference Panel strains. Overall design: 76 gut samples profiled from 38 DGRP strains under two conditions: control and infected with Pseudomonas entomophila.

Sample: DGRP-426_Control

SAMN09768063 • SRS3629699 • All experiments • All runs

Organism: Drosophila melanogaster

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: For each condition, guts from 30 adult female flies were collected in PBS four hours post treatment and directly homogenized in trizol (Invitrogen) using a bead-beater. A standard protocol for RNA extraction was performed. Illumina stranded Truseq libraries

Experiment attributes:

GEO Accession: GSM3319296

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 22.1M spots, 2.2G bases, 1.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR7647343	22,142,010	2.2G	1.3Gb	2019-10-07

ID:: 6107956

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