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SRX4924223: GSM3443292: ZEB1+/- -LV_3; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 30.4M spots, 1.6G bases, 986.7Mb downloads

Submitted by: NCBI (GEO)
Study: ZEB1 insufficiency causes corneal endothelial cell state transition and altered cellular processing
show Abstracthide Abstract
The zinc finger e-box binding homeobox 1 (ZEB1) transcription factor is a master regulator of the epithelial to mesenchymal transition (EMT), and of the reverse mesenchymal to epithelial transition (MET) processes. ZEB1 plays an integral role in mediating cell state transitions during cell lineage specification, wound healing and disease. EMT/MET are characterized by distinct changes in molecular and cellular phenotype that are generally context-independent. Posterior polymorphous corneal dystrophy (PPCD), associated with ZEB1 insufficiency, provides a new biological context in which to understand and evaluate the classic EMT/MET paradigm. PPCD is characterized by a cadherin-switch and transition to an epithelial-like transcriptomic and cellular phenotype, which we study in a cell-based model of PPCD generated using CRISPR-Cas9-mediated ZEB1 knockout in corneal endothelial cells (CEnCs). Transcriptomic and functional studies support the hypothesis that CEnC undergo an MET-like transition in PPCD, termed endothelial to epithelial transition (EnET), and lead to the conclusion that EnET may be considered a corollary to the classic EMT/MET paradigm. Overall design: Three independent clones for each genotype were generated. ZEB1+/+ and ZEB1+/- (generated using CRISPR-Cas9 gene editing) parental lines were initially generated, then transduced with lentivirus containing ZEB1 cDNA to generate ZEB1 transgenic lines of the parental lines.
Sample: ZEB1+/- -LV_3
SAMN10281399 • SRS3969500 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: TriReagent extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was further purified using the Qiagen RNeasy Clean-Up Kit. RNA-seq libraries were prepared using the KAPA mRNA HyperPrep Kit using an automated liquid handler (Janus G3 – PerkinElmer) according to manufacturer's instruction at the UCLA Institute for Quantitative and Computational Biology.
Experiment attributes:
GEO Accession: GSM3443292
Links:
Runs: 1 run, 30.4M spots, 1.6G bases, 986.7Mb
Run# of Spots# of BasesSizePublished
SRR809737930,441,0771.6G986.7Mb2019-06-14

ID:
6625583

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