Instrument: NextSeq 500
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: Basal lingual epithelial cells were purified from P0 newborn mice. Tongues were removed from the mandible and cut to exclude the posterior circumvallate papillae. For ChIP, only control mice were collected. Tissues were cut into small pieces and incubated with 1.26U/mL dispase (Invitrogen) and 0.3% type 1 collagenase (Worthington) for 45 minutes at 37ºC with 80 rpm shaking. Tissues were washed with 1xPBS, dissociated with 0.25% Trypsin with 2.21mM EDTA (Corning Cellgro; Manassas, VA, USA), and then washed twice with 1xPBS. Cells were stained with 1:200 EpCAM-APC antibodies (Biolegend; San Diego, CA, USA) for 30 min on ice and washed twice with 1x HBSS prior to cell sorting. For high-throughput ChIP sequencing, libraries were constructed from 4ng of Purified DNA using the DNA SMART ChIP-Seq Kit (Clontech; Palo Alto, CA, USA) according to the manufacturer's instructions. Constructed ChIP-seq libraries were sequenced on the Illumina NextSeq 500 platform. Two biological replicates were used for ChIP experiments.