GSM1377954: WT3_LSK; Mus musculus; RNA-Seq - SRA

SRX531216: GSM1377954: WT3_LSK; Mus musculus; RNA-Seq
1 ION_TORRENT (Ion Torrent Proton) run: 54.3M spots, 4.8G bases, 3.7Gb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Tet2-/-Flt3ITD and WT stem and progenitor cells

PRJNA246093 • SRP041625 • All experiments • All runs

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The study was a comparison of gene expression using RNA-seq. We analyzed the stem and progenitor cells from WT and Vav-cre+ Tet2fl/fl Flt3-ITD (T2F3) mice. We isolated stem cells LSK (lin- sca+ kit+) and granulocyte-macrophage progenitors GMP (lin- sca- kit+ fcgr+ cd34+) cells from bone marrow. Comparisons were made across genotypes WT vs. T2F3 and cell types LSK vs. GMP. Overall design: Comparison of WT and Tet2-/-Flt3ITD bone marrow stem and progenitor cells.

Sample: WT3_LSK

SAMN02742770 • SRS600384 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Ion Torrent Proton

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Cells were lysed directly in Trizol (Life Technologies). Follow Ion Torrent protocol, start with 100ng RNA using Ambion Low Input RiboMinus Eukaryote System v2 kit + LifeTech Ion Total RNA seq kit V2.

Experiment attributes:

GEO Accession: GSM1377954

Links:

External link: GEO Sample GSM1377954

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 54.3M spots, 4.8G bases, 3.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR1268229	54,283,438	4.8G	3.7Gb	2015-05-01

ID:: 731773

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