GSM3677461: myo>UASlark_Pe_4h_1; Drosophila melanogaster; RNA-Seq - SRA

SRX5537904: GSM3677461: myo>UASlark_Pe_4h_1; Drosophila melanogaster; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 47.7M spots, 7.6G bases, 2.9Gb downloads

Submitted by: NCBI (GEO)

Study: RNA-sequencing of the female adult fly guts with enterocyte-specific overexpression and silencing of lark in control and Pseudomonas entomophila infected states.

PRJNA527835 • SRP188752 • All experiments • All runs

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The lark gene was either overexpressed or silenced in adult enterocytes. Flies were fed sucrose or Pseudomonas entomophila (Pe). Overall design: The lark gene was either overexpressed or silenced in adult enterocytes. Flies were fed sucrose or Pseudomonas entomophila (Pe), Total RNA was extracted and paired-end sequencing was performed. The experiment was performed three times ( three biological replicates)

Sample: myo>UASlark_Pe_4h_1

SAMN11160938 • SRS4503718 • All experiments • All runs

Organism: Drosophila melanogaster

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: For each condition, guts from 30 adult female flies were collected in PBS four hours post treatment and directly homogenized in trizol (Invitrogen) using a bead-beater. A standard protocol for RNA extraction was performed. Paired-end Illumna stranded Truseq libraries

Experiment attributes:

GEO Accession: GSM3677461

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 47.7M spots, 7.6G bases, 2.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR8745884	47,740,963	7.6G	2.9Gb	2019-10-07

ID:: 7468601

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