GSM3746660: 6M_HBs-HepR, liver_rep5; Mus musculus; RNA-Seq - SRA

SRX5784297: GSM3746660: 6M_HBs-HepR, liver_rep5; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq X Ten) run: 33.3M spots, 10G bases, 3Gb downloads

Submitted by: NCBI (GEO)

Study: A novel mouse model of hepatocellular carcinoma mediated by hepatitis B virus-specific CD8+ T cells [bulk RNA-seq]

PRJNA540938 • SRP194744 • All experiments • All runs

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We used immunocompetent Fah-/- mice as the recipients and adoptively transferred HBsAg+ hepatocytes from HBs-Tg mice to replace the recipient hepatocytes (HBs-HepR). HBs-HepR mice maintained persistent HBsAg expression with chronic hepatitis and spontaneous liver fibrosis, and eventually developed HCC with a prevalence of 100%. Overall design: Liver tissue mRNA profiles of B6-HepR and HBs-HepR mice after 6 months and 12 months post transfer were generated by deep sequencing using Illumina

Sample: 6M_HBs-HepR, liver_rep5

SAMN11569404 • SRS4716991 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq X Ten

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: liver tissue were harvested, flash frozen in liquid nitrogen, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM3746660

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 33.3M spots, 10G bases, 3Gb

Run	# of Spots	# of Bases	Size	Published
SRR9005995	33,306,657	10G	3Gb	2023-04-21

ID:: 7779553

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