GSM3911310: ctrl_LPS_81; Homo sapiens; RNA-Seq - SRA

SRX6384188: GSM3911310: ctrl_LPS_81; Homo sapiens; RNA-Seq
2 ILLUMINA (NextSeq 500) runs: 44.9M spots, 3.6G bases, 1.3Gb downloads

Submitted by: NCBI (GEO)

Study: miR-221-3p drives the Shift of M2-Macrophage to a Pro-Inflammatory Function by Modulating JAK3/STAT3 Signaling and is Increased in Rheumatoid Arthritis

PRJNA551688 • SRP212629 • All experiments • All runs

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We performed a transcriptome analysis of M2 macrophages generated in vitro from monocytes isolated from peripheral blood of healthy donors, and studied the effect of miR-221-3p mimic transfection and stimulation with LPS. Overall design: miR-221-3p mimic was added to cultured macrophages for 63.5 hours, prior to stimulation with 100 ng/ml LPS for 8.5 hours.

Sample: ctrl_LPS_81

SAMN12163114 • SRS5043706 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNA for the 20 resulting samples was isolated using miRNeasy Micro kit (Qiagen). RNA quality was assessed with a Fragment Analyzer (Advanced Analytical). Library preparation was performed using the Illumina Truseq stranded kit.

Links:

NCBI link: NCBI Entrez (gds)

Runs: 2 runs, 44.9M spots, 3.6G bases, 1.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR9621284	23,957,565	1.9G	714.3Mb	2020-01-15
SRR9621285	20,904,906	1.7G	602.2Mb	2020-01-15

ID:: 8457256

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