Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Embryos were decorionated and deyolked in DMEM with high Glucose, no Glutamine, no Calcium (11530556, Gibco); hindbrains were micro-dissected using 0.33mm micro-fine sterile needles; RNA was isolated using Quick-RNA Microprep kit (Zymo Research) Samples were prepared with the Ovation® RNA-Seq System V2 (7102, NuGEN) for cDNA amplification, followed by NexteraXT (Illumina) for library preparation.