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SRX7748673: GSM4320178: Stimulated_M1_18h_3; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 3000) run: 9M spots, 1.4G bases, 472.4Mb downloads

Submitted by: NCBI (GEO)
Study: Triacylglycerol synthesis enhances macrophage inflammatory function
show Abstracthide Abstract
Macrophages stimulated with lipopolysaccharide (LPS) plus interferon-g (IFNg) accumulate TGs in LDs, and long-chain acylcarnitines. Inhibition of TG synthesis results in diminished LD development, and increased long chain acylcarnitine levels, suggesting that FA fate is balanced between TG and acylcarnitine synthesis. Nevertheless, TG-synthesis is required for inflammatory macrophage function, since its inhibition negatively affects production of proinflammatory IL-1b, IL-6 and PGE2, and phagocytic capacity, and protects against LPS-induced sock in vivo. Overall design: RNA-Seq was performed on resting or activated Macrophages treated with or without the DGAT1 inhibitor T863
Sample: Stimulated_M1_18h_3
SAMN14134890 • SRS6167975 • All experiments • All runs
Organism: Mus musculus
Instrument: Illumina HiSeq 3000
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was isolated using the PureLink RNA Mini kit (Ambion) and quantified using Qubit 2.0. Libraries were prepared using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced using the HiSeq 3000 (Illumina).
Experiment attributes:
GEO Accession: GSM4320178
Runs: 1 run, 9M spots, 1.4G bases, 472.4Mb
Run# of Spots# of BasesSizePublished


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