GSM4338501: RPMI_8226_ChIP_Input; Homo sapiens; ChIP-Seq - SRA

SRX7798966: GSM4338501: RPMI_8226_ChIP_Input; Homo sapiens; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 49.8M spots, 2.5G bases, 1.9Gb downloads

Submitted by: NCBI (GEO)

Study: H3K27ac ChIP-seq for multiple myeloma cell lines, B-lymphoma cells and normal plasma cell

PRJNA608682 • SRP250673 • All experiments • All runs

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ChIP-seq analysis was performed in Multiple Myeloma cell lines to analyze acetylation of histone H3K27ac. Overall design: Daudi, RAJI, H929, JJN3, KMS11, KMS12, KMS28BM, RPMI8226, U266 and NP (normal plasma cell) were crosslinked with formaldehyde for 10 min. DNA was enriched by chromatin-immunoprecipitation (ChIP) and analyzed by Illumina Hiseq 4000. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against histone H3 lysine 27 acetylation (H3K27Ac: Abcam, ab4729).

Sample: RPMI_8226_ChIP_Input

SAMN14206198 • SRS6213843 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 4000

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Construction protocol: Library construction from the H3K27ac ChIP-seq samples and sequencing of single-end 50bps long reads by the Illumina HiSeq 4000 were performed at BGI Biotech Solutions Co. Ltd (Hong Kong, China). Single end ChIP-Seq sequencing

Experiment attributes:

GEO Accession: GSM4338501

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 49.8M spots, 2.5G bases, 1.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR11178317	49,849,808	2.5G	1.9Gb	2021-02-16

ID:: 10201982

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