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SRX7798967: GSM4338502: U266_H3K27ac_ChIP-Seq; Homo sapiens; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 59.8M spots, 3G bases, 2.4Gb downloads

Submitted by: NCBI (GEO)
Study: H3K27ac ChIP-seq for multiple myeloma cell lines, B-lymphoma cells and normal plasma cell
PRJNA608682 • SRP250673 • All experiments • All runs
show Abstracthide Abstract
ChIP-seq analysis was performed in Multiple Myeloma cell lines to analyze acetylation of histone H3K27ac. Overall design: Daudi, RAJI, H929, JJN3, KMS11, KMS12, KMS28BM, RPMI8226, U266 and NP (normal plasma cell) were crosslinked with formaldehyde for 10 min. DNA was enriched by chromatin-immunoprecipitation (ChIP) and analyzed by Illumina Hiseq 4000. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against histone H3 lysine 27 acetylation (H3K27Ac: Abcam, ab4729).
Sample: U266_H3K27ac_ChIP-Seq
SAMN14206197 • SRS6213844 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 4000
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: Library construction from the H3K27ac ChIP-seq samples and sequencing of single-end 50bps long reads by the Illumina HiSeq 4000 were performed at BGI Biotech Solutions Co. Ltd (Hong Kong, China). Single end ChIP-Seq sequencing
Experiment attributes:
GEO Accession: GSM4338502
Links:
Runs: 1 run, 59.8M spots, 3G bases, 2.4Gb
Run# of Spots# of BasesSizePublished
SRR1117831859,755,6973G2.4Gb2021-02-16

ID:
10201983

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