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SRX7798977: GSM4338512: MM5_H3K27ac_ChIP-seq; Homo sapiens; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 57.3M spots, 2.9G bases, 2.3Gb downloads

Submitted by: NCBI (GEO)
Study: H3K27ac ChIP-seq for primary multiple myeloma patients' samples (MM1-MM10)
PRJNA608681 • SRP250674 • All experiments • All runs
show Abstracthide Abstract
ChIP-seq analysis was performed in primary Multiple Myeloma patients' samples to analyze acetylation of histone H3K27ac. Overall design: Primary multiple myeloma patients' samples (MM1-MM10) were crosslinked with formaldehyde for 10 min. DNA was enriched by chromatin-immunoprecipitation (ChIP) and analyzed by Illumina Hiseq 4000. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against histone H3 lysine 27 acetylation (H3K27Ac: Abcam, ab4729).
Sample: MM5_H3K27ac_ChIP-seq
SAMN14206187 • SRS6213854 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 4000
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: Library construction from the H3K27ac ChIP-seq samples and sequencing of single-end 50bps long reads by the Illumina HiSeq 4000 were performed at BGI Biotech Solutions Co. Ltd (Hong Kong, China). Single end ChIP-Seq sequencing
Experiment attributes:
GEO Accession: GSM4338512
Links:
Runs: 1 run, 57.3M spots, 2.9G bases, 2.3Gb
Run# of Spots# of BasesSizePublished
SRR1117832857,294,0502.9G2.3Gb2021-02-16

ID:
10201993

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