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SRX8111221: GSM4474575: Larynx, LH30; Oryctolagus cuniculus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 93.3M spots, 27.7G bases, 8.7Gb downloads

Submitted by: NCBI (GEO)
Study: RNA-Seq of vocal folds of rabbits exposed to a low relative humidity environment
show Abstracthide Abstract
The purpose of this study is to investigate if acute mucosal dehydration as a funcition of low relative humidty environment impacts the vocal folds at the mRNA level. Rabbits were used as animal model to enable a systematic and rigorous assessment of the pathobiology of vocal fold hydration since they can be manipulated in a physiologically realistic manner. We anticipate that the results of this study will help to elucidate the effects of altered mucosal hydration state in response to low ambient humidity on vocal fold epithelium, lamina propria and muscle tissue at the transcriptome level. Together with cellular, structural, and functional techniques that are part of this project, we expect to provide validated scientific recommendations on the adverse effects of mucosal dehydration and the therapeutic benefits of hydration. Overall design: The transcriptome of vocal folds of rabbits that were exposed to low relative humidity (n=6) were compared to those from rabbits held at a standard ambient humidity (n=6).
Sample: Larynx, LH30
SAMN14592580 • SRS6476295 • All experiments • All runs
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA of vocal folds (low humidity n=6, moderate humidity n=6) was extracted with commercial kit RNeasy Fibrous Tissue Mini Kit (QIAGEN). Library construction was done using NuGEN's Universal Plus mRNA Kit. Each library was performed with 200 ng of RNA. Unique barcodes were used for each library.
Experiment attributes:
GEO Accession: GSM4474575
Runs: 1 run, 93.3M spots, 27.7G bases, 8.7Gb
Run# of Spots# of BasesSizePublished


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