GSM4509854: ctrl_rep3; Homo sapiens; RNA-Seq - SRA

SRX8241503: GSM4509854: ctrl_rep3; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 550) run: 28.4M spots, 2.8G bases, 1.4Gb downloads

Submitted by: NCBI (GEO)

Study: Structural interaction between DISC1 and ATF4 underlying transcriptional and synaptic dysregulation in an iPSC model of mental disorders

PRJNA629813 • SRP259940 • All experiments • All runs

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Analysis of gene expression alteration in iPSC-derived cortical neurons with ATF4 overexpression Overall design: Total RNA obtained from 4-week-old forebrain neurons derived from C3 and C3 with ATF4 over-expression.

Sample: ctrl_rep3

SAMN14791882 • SRS6591142 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: NextSeq 550

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was extracted with mirVana kit (Invitrogen) in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser. Libraries were generated from 1 μg of total RNA from three biological replicates of 4-week-old forebrain neurons using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB) following the manufacturer's protocol.

Experiment attributes:

GEO Accession: GSM4509854

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 28.4M spots, 2.8G bases, 1.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR11680643	28,387,725	2.8G	1.4Gb	2020-05-07

ID:: 10743126

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