GSM4563710: HepG2_fixed61; Homo sapiens; RNA-Seq - SRA

SRX8377057: GSM4563710: HepG2_fixed61; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 1.9M spots, 288.7M bases, 119Mb downloads

Submitted by: NCBI (GEO)

Study: Effect of methanol fixation on single-cell RNA sequencing data

PRJNA634342 • SRP262635 • All experiments • All runs

show Abstracthide Abstract

We report how methanol fixation influences transcriptome profile in single cell RNA-seq. We generatad Smart-seq2 data from two cell lines, and both live and fixed cells from each cell line were processed and analyzed to illustrate fixaiton effect. Overall design: We used Smart-seq2 to generate single cell RNA-seq data from two cell lines: HCT-116 and HepG2. Both live and fixed cells were generated from each cell line.

Sample: HepG2_fixed61

SAMN14992124 • SRS6691615 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: cDNA library was constructed using Smart-seq2 protocl, sequencing library was prepared using Illuminar Nextera DNA Library Prep Kit(Illumina, cat#FC-131-1096), we followed library contruction protocal provided by C1 Fluidigm(Fluidigm)

Experiment attributes:

GEO Accession: GSM4563710

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 1.9M spots, 288.7M bases, 119Mb

Run	# of Spots	# of Bases	Size	Published
SRR11826324	1,921,782	288.7M	119Mb	2021-05-19

ID:: 10894671

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