GSM4745858: MM.1S BR-Input; Homo sapiens; ChIP-Seq - SRA

SRX9006276: GSM4745858: MM.1S BR-Input; Homo sapiens; ChIP-Seq
1 ILLUMINA (HiSeq X Ten) run: 18.4M spots, 5.5G bases, 2.1Gb downloads

Submitted by: NCBI (GEO)

Study: Targeting steroid receptor co-activator 3 sensitizes myeloma cell to proteasome inhibitor treatment through NSD2-mediated phase separation and chromatin remodeling [ChIP-Seq]

PRJNA659375 • SRP278913 • All experiments • All runs

show Abstracthide Abstract

Functional crosstalk between histone modifications and chromatin remodeling has emerged as a key regulatory mode of transcriptional control during cell fate decisions, but the underlying mechanisms are not fully understood. Here we demonstrate that NSD2/SRC-3 complex coordinates histone H3 lysine 36 dimethylation (H3K36me2) and transcriptional elongation factor Pol II to regulate chromatin dynamic and gene transcription during myeloma resistant to bortezomib. Mechanistically, NSD2 can interact with SRC-3, its SET domain is responsible to H3K36me2 to enhance the transcriptional activity of SRC-3 target gene. The inhibitor of SRC-3, SI-2, can impaired the interaction between NSD2 and SRC-3, caused the distribution of H3K36me2 on the genome-wide. Overall design: Examination of histone modification in multiple myeloma bortezomib-resistant cell line

Sample: MM.1S BR-Input

SAMN15904186 • SRS7267019 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: HiSeq X Ten

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: PAIRED

Construction protocol: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to the TruePrep® DNA Library Prep Kit V2 for Illumina (TD503-02). Briefly, TruePrep Tagment Enzyme Mix (TTEMix) contains transposase and two kinds of adapters (Adapter 1 and Adapter 2) with equal molar. Input DNA are fragmented and linked with adapters on both ends just by mixing with TTE Mix, followed by a 10-minute incubation at 55℃. The tagged DNA fragments can be further amplified with two pairs of primers N5 (N5XX) / N7 (N7XX) and P5 / P7 (PCR Primer Mix, PPM). After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina HiSeq X following the manufacturer's protocols.

Experiment attributes:

GEO Accession: GSM4745858

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 18.4M spots, 5.5G bases, 2.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR12515748	18,423,268	5.5G	2.1Gb	2020-08-27

ID:: 11705321

SRA

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