DNA-seq of Rat:CDr/y - SRA

SRX1483180: DNA-seq of Rat:CDr/y
1 ILLUMINA (Illumina HiSeq 2500) run: 599.6M spots, 121.1G bases, 52.7Gb downloads

Design: We performed DNA-seq PCR-free as previously described (Blecher-Gonen, Barnett-Itzhaki, Jaitin, Amann-Zalcenstein, Lara-Astiaso, and Amit 2013), with the following modifications: Two samples of ~1ug of genomic Rat liver DNA were sheared to a peak of 291 and 286bp, using the Covaris S220 sonicator (Covaris, Inc., Woburn, MA, USA). We performed end repair in 80ul reaction at 20oC for 30min. Following cleanup with Agencourt AmPURE XP beads (Beckman Coulter, Inc., Indianapolis, IN, USA) at a ratio of 0.75X Beads/DNA volume, we added bases to both 3 ends. We ligated adapters (Bioo Scientific Cat# 514112) in a final concentration of 1.1uM following a SPRI beads cleanup at a ratio of 0.75x beads/DNA volume. Sample preparation was without the PCR step. Libraries quantitation was in 50 Single Read HigSeq runs.

Submitted by: Weizmann Institute of Science

Study: Dissection of the genetic basis of diet-induced type 2 diabetes the Cohen Diabetic rat

PRJNA305815 • SRP067304 • All experiments • All runs

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We sequenced (NGS) the genomes of the Cohen Diabetic sensitive (CDs/y) and the Cohen Diabetic resistant (CDr/y) strains, a fully inbred model of diet-induced type 2 diabetes, in order to detect sequence variations of potential relevance to the diabetic phenotype.

Sample:

SAMN04334489 • SRS1204746 • All experiments • All runs

Organism: Rattus norvegicus

Library:

Name: 2

Instrument: Illumina HiSeq 2500

Strategy: WGA

Source: GENOMIC

Selection: unspecified

Layout: PAIRED

Spot descriptor:

1 forward102 reverse

Pipeline: show...hide...

Program	Version
BWA	0.7.5a-r405

Runs: 1 run, 599.6M spots, 121.1G bases, 52.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR3002495	599,607,189	121.1G	52.7Gb	2016-08-08

ID:: 2093522

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