U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX1164554: Methylome of the Norway rat Rn_G1_methylome
1 ILLUMINA (Illumina HiSeq 2000) run: 504.5M spots, 77G bases, 45.9Gb downloads

Design: 5 ug genomic DNA was fragmented into a mean size of ~250 bp, followed by end repair, 3'-end addition of dA and adapter ligation. Methylated adapters were used according to the manufacturer's instructions (Illumina). The bisulfite conversion of sample DNA was carried out using a modified NH4HSO3-based protocol 1 and amplified with 9 cycles of PCR. Paired-end sequencing (PE 91) was carried out using an illumina HiSeq2000.
Submitted by: Institute of Plant Protection, Chinese Academy of
Study: Rattus norvegicus genome resequencing
show Abstracthide Abstract
Through profiling of the sperm methylomes and genomes of two rat subspecies from South and North China, we characterized the impact of transgenerational epigenetic inheritance on the evolvability or evolutionary rate of the DNA
Sample:
SAMN03995893 • SRS1045311 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2000
Strategy: Bisulfite-Seq
Source: GENOMIC
Selection: unspecified
Layout: PAIRED
Spot descriptor:
forward91  reverse

Runs: 1 run, 504.5M spots, 77G bases, 45.9Gb
Run# of Spots# of BasesSizePublished
SRR3095877504,464,07777G45.9Gb2017-06-23

ID:
1694657

Supplemental Content

Search details

See more...

Recent activity