ale yeast 454 reads - SRA

SRX1037572: ale yeast 454 reads
1 LS454 (454 GS FLX Titanium) run: 609,027 spots, 213.8M bases, 141.9Mb downloads

Design: To minimize the amount of mitochondrial DNA during sequencing, GSY2239 was plated on YEP-glucose containing 30 ug/ml of ethidium bromide, a protocol that is known to induce petite mutations. After two days, multiple colonies were isolated and lack of growth on a non-fermentable substrate (YEP-glycerol) was confirmed. For genomic DNA extraction, a single colony, GSY2239.1 was grown overnight in 3 ml YEP + 2% dextrose. Cells were resuspended in 200 ul Triton X-100, 1% SDS, 100 mM NaCL, 50 mM Tris-Cl (pH 8.0) and 1 mM EDTA (pH 8.0) whereby DNA was extracted using phenol:chloroform and resuspended in 200 ul TE RNAse (50 ug/ml).

Submitted by: UNIVERSITY OF ARIZONA

Study: Saccharomyces cerevisiae strain:GSY2239 Genome sequencing and assembly

PRJNA284803 • SRP058662 • All experiments • All runs

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Whole genome sequencing of the ale-producing yeast Saccharomyces cerevisiae strain GSY2239 (Duvel Moortgat Brewery, Belgium)

Sample: Saccharomyces cerevisiae strain GSY2239 laboratory isolate

SAMN03733270 • SRS944788 • All experiments • All runs

Organism: Saccharomyces cerevisiae

Library:

Name: ale yeast wga library

Instrument: 454 GS FLX Titanium

Strategy: WGA

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Spot descriptor:

5 forward

Runs: 1 run, 609,027 spots, 213.8M bases, 141.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR2039176	609,027	213.8M	141.9Mb	2015-06-18

ID:: 1504384

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