Whole Genome Sequencing of Saccharomyces cerevisiae from New Zealand:... - SRA

SRX553994: Whole Genome Sequencing of Saccharomyces cerevisiae from New Zealand: Sample 35-HB_S_GIMBLETTROAD_5
1 ILLUMINA (Illumina HiSeq 2000) run: 2.5M spots, 738.5M bases, 493.1Mb downloads

Design: Genomic DNA was sheared into smaller fragments of a desired size by a Covaris E210. The overhangs resulting from fragmentation were converted into blunt ends by using T4 DNA polymerase, Klenow Fragment and T4 Polynucleotide Kinase. After adding an "A" base to the 3' end of the blunt phosphorylated DNA fragments, adapters were ligated to the ends of the DNA fragments. The desired fragments were purified though gel-electrophoresis, then selectively enriched and amplified by PCR. The index tag was added to the adapter at the PCR stage.

Submitted by: The University of Auckland

Study: New Zealand Saccharomyces cerevisiae genome sequencing

PRJNA247448 • SRP042301 • All experiments • All runs

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The purpose of this project is to determine the origins, diversity and ancestry of Saccharomyces cerevisiae in New Zealand using whole genome sequencing.

Sample: Saccharomyces cerevisiae HB_S_GIMBLETTROAD_5

SAMN02776123 • SRS621280 • All experiments • All runs

Organism: Saccharomyces cerevisiae

Library:

Name: 35-HB_S_GIMBLETTROAD_5

Instrument: Illumina HiSeq 2000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Spot descriptor:

1 forward151 reverse

Runs: 1 run, 2.5M spots, 738.5M bases, 493.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR1300186	2,461,682	738.5M	493.1Mb	2015-07-22

ID:: 791338

SRA

Sequence Read Archive

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