show Abstracthide AbstractTill now no comprehensive attempt has been made to genotype rich diversity of Indian silkworm breeds in such a way to identify each of them. NGS technologies have made it easier to develop methodologies to generate SNPs that can identify strains and can be used to select contrasting parents in breeding programs. Here we propose to detect SNP variation in silkworm by Genotyping by sequencing (GBS). The SNP information will also enable for association or linkage analysis of traits of commercial interest in silkworm.