Creating phylogenetic tree in Genome Workbench from a multiple alignment

For fast/rough tree construction use newly created multiple alignment, or upload alignment (in Fasta format) created previously (using File -> Open -> select File Import, for File Format select FASTA Alignment files, browse to your file and click Next/Next/Finish) into new project.

Select multiple alignment file in the Project view, open Tools dialog, select Phylogenetic Tree Builder Tool, and click Next:

Run Phylogenetic Tree Builder Tool

In the “Run Tool – Phylogenetic Tree Builder Tool” dialog select alignment (if not selected by default), and select Distance Method (Poisson (protein), Kimura (protein) or Jukes Cantor (DNA)), Tree Construct Method (Neighbor Joining or Fast Minimum Evolution) and Labels for Leaf Nodes representation, and click Finish.

Tree Builder Tool Options

Wait for tree construction (might take a few minutes, depending on the alignment size). Find newly created tree and distant matrix added to the project:

Newly Created Tree

Select Tree file in the project view and open in Tree View.

Open Tree View

Tree opens at zoom 0:

Tree Zoom 0

Open right click menu and see set of options that can be used for tree manipulation. For our tree we used: layout - rectangle cladogram, zoom behavior - vertical, re-root - set midpoint root. For the leaf node labels we select settings/labels/custom labels using properties and set up $(label)__$(organism) in this “Properties” dialog:

Tree Properties

For complete set of tree manipulation/editing options see Using Tree View tutorial.

Pease also see the Phylogenetic Trees Video tutorial.

In tree view, zoom in to the level where individual labels are seen and see custom labels:

Zoom To Labels

Current Version is 3.6.0 (released March 04, 2021)

Release Notes





General use Manuals

NCBI GenBank Submissions Manuals

Other Resources

Support Center

Last updated: 2020-08-28T13:32:31Z