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| Status |
Public on Sep 30, 2021 |
| Title |
Transcriptional profiling of the Candida auris response to exogenous farnesol exposure |
| Organism |
[Candida] auris |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The antifungal resistance threat posed by Candida auris necessitates bold and innovative therapeutic options. Farnesol, a quorum-sensing molecule with a potential antifungal and/or adjuvant effect; it may be a promising candidate in alternative treatment regimens. To gain further insights into the farnesol-related effect on C. auris, genome-wide gene expression analysis was performed using RNA-Seq. Farnesol exposure resulted in 1,766 differentially expressed genes. Of these, 447 and 304 genes with at least 1.5-fold increase or decrease in expression, respectively, were selected for further investigation. Genes involved in morphogenesis, biofilm events (maturation and dispersion), gluconeogenesis, iron metabolism, and regulation of RNA biosynthesis showed down-regulation, whereas those related to antioxidative defense, transmembrane transport, glyoxylate cycle, fatty acid β-oxidation, and peroxisome processes were up-regulated. In addition, farnesol treatment increased the expression of certain efflux pump genes, including MDR1, CDR1, and CDR2. Growth, measured by change in CFU number, was significantly inhibited within 2 hours of the addition of farnesol (5.8×10^7±1.1×10^7 and 1.1×10^7±0.3×10^7 CFU/ml for untreated control and farnesol-exposed cells, respectively) (p<0.001). In addition, farnesol treatment caused a significant reduction in intracellular iron (152.2±21.1 vs. 116.0±10.0 mg/kg), manganese (67.9±5.1 vs. 18.6±1.8 mg/kg), and zinc (787.8±22.2 vs. 245.8±34.4 mg/kg) (p<0.05–0.001) compared to untreated control cells, whereas the level of cooper was significantly increased (274.6±15.7 vs. 828.8±106.4 mg/kg) (p<0.001). Our data demonstrate that farnesol significantly influences the growth, intracellular metal ion contents, and gene expression related to fatty acid metabolism, which could open new directions in developing alternative therapies against C. auris.
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| Overall design |
Examination of untreated control and farnesol-treated Candida auris cultures in three replicates.
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| Contributor(s) |
Kovács R, Jakab Á |
| Citation(s) |
34643421 |
| Submission date |
Jul 14, 2021 |
| Last update date |
Nov 10, 2021 |
| Contact name |
Agnes Jakab |
| E-mail(s) |
jakab.agnes@med.unideb.hu
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| Organization name |
University of Debrecen
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| Street address |
Nagyerdei krt 98.
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| City |
Debrecen |
| ZIP/Postal code |
4032 |
| Country |
Hungary |
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| Platforms (1) |
| GPL24811 |
Illumina NextSeq 500 ([Candida] auris) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA746543 |
| SRA |
SRP328312 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE180093_RNASeq.xlsx |
1.1 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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