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Series GSE18039 Query DataSets for GSE18039
Status Public on Feb 21, 2010
Title MicroRNAs show diverse and dynamic expression patterns in multiple tissues of Bombyx mori
Organism Bombyx mori
Experiment type Non-coding RNA profiling by array
Summary Background: MicroRNAs (miRNAs) repress target genes at the post-transcriptional level, and function in the development and cell-lineage pathways of host species. Tissue-specific expression of miRNAs is highly relevant to their physiological roles in the corresponding tissues. However, to date, few miRNAs have been spatially identified in the silkworm.
Results: We establish for the first time the spatial expression patterns of nearly 100 miRNAs in multiple normal tissues (organs) of Bombyx mori females and males using microarray and Northern-blotting analyses. In total, only 10 miRNAs were universally distributed (including bmo-let-7 and bmo-bantam), while the majority were expressed exclusively or preferentially in specific tissue types (e.g. bmo-miR-275 and bmo-miR-1). We additionally examined the developmental patterns of miRNA expression during metamorphosis of the body wall, silk glands, midgut and fat body. In total, 63 miRNAs displayed significant alterations in abundance in at least 1 tissue during the developmental transition from larvae to pupae (e.g. bmo-miR-263b and bmo-miR-124). Expression patterns of five miRNAs were significantly increased during metamorphosis in all four tissues (e.g. bmo-miR-27 and bmo-miR-305).
Conclusions: In this study, we conducted preliminary spatial measurements of several miRNAs in the silkworm. Periods of rapid morphological change were associated with alterations in miRNA expression patterns in the body wall, silk glands, midgut and fat body during metamorphosis. Accordingly, we propose that this ubiquitous or tissue-specific expression of miRNAs supports their critical roles in tissue specification. The results obtained should facilitate future functional analyses.
 
Overall design To determine the global spatial expression patterns of miRNAs in silkworm, we designed a DNA oligonucleotide-based microarray examining 92 unique miRNAs with 106 antisense probes. To determine the extent of tissue-specific changes during the specific developmental events, we assessed changes in miRNA expression in four individual tissues and organs (body wall, silk glands, midgut and fat body) from the larval to pupal stages.
 
Contributor(s) Liu S, Gao S, Zhang D, Yin J, Xiang Z, Xia Q
Citation(s) 20122259
Submission date Sep 09, 2009
Last update date Mar 21, 2012
Contact name Qingyou Xia
E-mail(s) xiaqy@swu.edu.cn
Phone 086-023-68250099
Fax 086-02368251128
Organization name Southwest University
Department College of Biotechnology
Lab The Key Sericultural Laboratory of Agricultural Ministry
Street address Tiansheng Road
City Beibei
State/province Chongqing
ZIP/Postal code 086-400716
Country China
 
Platforms (1)
GPL9166 CapitalBio Silkworm microRNA _microarray Release 1
Samples (64)
GSM451052 head of female silkworm_HD(f)-a, group 1
GSM451053 head of female silkworm_HD(f)-b, group 1
GSM451054 head of male silkworm_HD(m)-a, group 1
Relations
BioProject PRJNA119897

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE18039_RAW.tar 7.2 Mb (http)(custom) TAR (of GPR, JPG, TXT)
Processed data included within Sample table
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