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| Status |
Public on Jul 26, 2011 |
| Title |
Expression data from mouse skin scar after wounding |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Using a TCF reporter mouse which contain a LacZ gene downstream of a c-fos minimal promoter and three consensus TCF-binding motifs, we wounded skin and harvested the scar tissue one week post wounding. Healing tissue sorted to group of cells : β-Gal+ cells (R) expression data with β-Gal- cells (L) , an analysis of gene expression was performed using Partek Genotyping Suite and Ingenuity Systems Software.
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| Overall design |
We used microarrays to explore the difference in gene expression of B-Gal+ cells compare to Negative cells.Microdissected wound skin tissue was homogenized using a metal tissue smasher which pre-cooled in liquid nitrogen. Total RNA was extracted using Qiagen kit according to manufacturer’s manual. For microarray, RNA was extracted as mentioned and the quality of RNA was assessed with a Bioanalyzer (Agilent Technologies). cDNA was generated and hybridized onto the Affymetrix Mouse Gene 1.0 ST chips.
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| Contributor(s) |
Saeid A, Benjamin A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 25, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Saeid Amini-Nik |
| E-mail(s) |
saeid.amininik@utoronto.ca
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| Phone |
416-813-7231
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| Organization name |
Hospital For Sick Children
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| Street address |
101 College St
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| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (2) |
| GSM766346 |
B-Gal - cells from healing skin of TCF mouse |
| GSM766347 |
B-Gal + cells from healing skin TCF mouse |
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| Relations |
| BioProject |
PRJNA146307 |
