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| Status |
Public on Sep 03, 2011 |
| Title |
Very small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Very small embryonic-like (VSEL) cells have been described as putatively pluripotent stem cells present in murine bone marrow and human umbilical cord blood (hUCB) and as such are of high potential interest for regenerative medicine. However, there remain some questions concerning the precise identity and properties of VSEL cells, particularly those derived from hUCB. For this reason, we have carried out an extensive characterisation of purified populations of VSEL cells from a large number of UCB samples. Consistent with a previous report, we find that VSEL cells are CXCR4+, have a high density, are indeed significantly smaller than HSC and have an extremely high nuclear/cytoplasmic ratio. Their nucleoplasm is unstructured and stains strongly with Hoechst 33342. A comprehensive FACS screen for surface markers characteristic of embryonic, mesenchymal, neuronal or hematopoietic stem cells revealed negligible expression on VSEL cells. These cells failed to expand in vitro under a wide range of culture conditions known to support embryonic or adult stem cell types and a microarray analysis revealed the transcriptional profile of VSELs to be clearly distinct both from well-defined populations of pluripotent and adult stem cells and from the mature hematopoietic lineages. Finally, we detected an aneuploid karyotype in the majority of purified VSEL cells by fluorescence in situ hybridisation. These data support neither an embryonic nor an adult stem cell like phenotype, suggesting rather that hUCB VSEL are an aberrant and inactive population that is not comparable to murine VSEL.
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| Overall design |
VSEL cells (Lin-CD45-CXCR4+CD34-) and CD34+ VSEL cells (Lin-CD45-CXCR4+CD34+) from three individual fresh cord blood units were double sorted to highest purity and subjected to array analysis.
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| Contributor(s) |
Danova-Alt R, Heider A, Egger D, Cross M, Alt R |
| Citation missing |
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| Submission date |
Sep 02, 2011 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Andreas Heider |
| E-mail(s) |
aheider@trm.uni-leipzig.de
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| Organization name |
Universität Leipzig
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| Department |
Biologie
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| Lab |
TRM Leipzig
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| Street address |
Semmelweisstr. 14
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| City |
Leipzig |
| State/province |
Bayern |
| ZIP/Postal code |
04103 |
| Country |
Germany |
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| Platforms (1) |
| GPL13667 |
[HG-U219] Affymetrix Human Genome U219 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA145069 |
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