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| Status |
Public on May 31, 2014 |
| Title |
Expression data from different photosynthetic rate in Populus. |
| Platform organism |
Populus sp. |
| Sample organism |
(Populus alba x Populus glandulosa) x Populus tomentosa |
| Experiment type |
Expression profiling by array
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| Summary |
Despite the significance of nucleus genes in plant growth and development, little is known of the molecular bases of regulation of photosynthesis in woody plants.Hence discovering the genetic basis for photosynthesis related phenotypic variation and identifying the major genes controlling these complex traits in trees is essential.
Combining the microarray technique and bulked segregant analysis strategy, we used poplar as a model to detect the nucleus genes differentially expressed in segregation population of photosynthesis traits. We measured the F1 interspecific population and segregated the stable extrem samples into two groups including three pools containing five incividuals.Use the Affymetrix poplar gene chip to decrypt the gene functions and mechanisms in different photosynthetic rate.
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| Overall design |
Leaves were taken from high and low photosynthetic rate individuals for RNA extraction and hybridization on Affymetrix microarrays. H_A, H_B, H_C are from the high photosynthetic rate individuals. L_D,L_E, L_F are from the low photosynthetic rate incividuals.
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| Contributor(s) |
Wang B, Zhang D, Du Q |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 20, 2013 |
| Last update date |
Jan 02, 2015 |
| Contact name |
Bowen Wang |
| E-mail(s) |
wbw1011@yeah.net
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| Organization name |
Beijing Forest University
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| Street address |
Beijing Forest University
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| City |
Beijing |
| ZIP/Postal code |
100083 |
| Country |
China |
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| Platforms (1) |
| GPL4359 |
[Poplar] Affymetrix Poplar Genome Array |
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| Samples (6)
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| GSM1144811 |
High photosynthetic rate gene pool,H_A,biological rep1 |
| GSM1144812 |
High photosynthetic rate gene pool,H_B,biological rep2 |
| GSM1144813 |
High photosynthetic rate gene pool,H_C,biological rep3 |
| GSM1144814 |
Low photosynthetic rate gene pool,L_D,biological rep1 |
| GSM1144815 |
Low photosynthetic rate gene pool,L_E,biological rep2 |
| GSM1144816 |
Low photosynthetic rate gene pool,L_F,biological rep3 |
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| Relations |
| BioProject |
PRJNA203560 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE47105_RAW.tar |
23.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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