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| Status |
Public on Aug 07, 2015 |
| Title |
Gene expression profile (GEP) of myb-silenced CD34+ HPCs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The transcription factor cMyb plays a key role in human primary CD34+ hematopoietic progenitor cells (HPCs) lineage choice, by enhancing erythropoiesis at the expense of megakaryopoiesis. We previously demonstrated that cMyb affects erythroid versus megakaryocyte lineage decision in part by transactivating KLF1 and LMO2 expression. To further unravel the molecular mechanisms through which cmyb affects lineage fate decision, we profiled the miRNA and mRNA changes in myb-silenced CD34+ HPCs. mRNA and miRNA expression for each sample were profiled by Affymetrix GeneAtlas U219 strip array and Exiqon Human miRNome PCR Panel, respectively. miRNA/mRNA data were integrated by Ingenuity Pathway Analysis.
The integrative analysis of miRNA/mRNA expression changes upon c-myb silencing in human CD34+ HPCs highlighted a set of 19 miRNA with 150 anticorrelated putative target mRNAs. Among the miRNAs downregulated in myb-silenced progenitors with the highest number of predicted target mRNAs, we selected hsa-miR-486-3p based on the in vitro effects of its overexpression on HPCs commitment. Indeed, morphological and flow cytometric analyses after liquid culture showed that hsa-miR-486-3p overexpression in HPCs enhanced erythroid and granulocyte differentiation while restraining megakaryocyte and macrophage differentiation. Moreover, collagen-based clonogenic assay demonstrated a strong impairement megakaryocyte commitment upon hsa-miR-486-3p overexpression in CD34+ cells. Gene expression profiling of hsa-miR-486-3p overexpressing CD34+ cells enabled us to identify a set of 8 genes downregulated and computationally predicted, putative hsa-miR-486-3p targets. Among them, we selected c-maf transcript as upregulated upon myb silencing. Worth of note, c-maf silencing in CD34+ progenitor cells was able to reverse the affects of myb silencing on erythroid versus megakaryocyte lineage choice. Integrative miRNA/mRNA analysis highlighted a set of miRNAs and anticorrelated putative target mRNAs modulated upon myb silencing, therefore potential players in myb-driven HPCs lineage choice. Among them, we demonstrated the hsa-miR-486-3p/c-maf pair as partially contributing to the effects of myb on HPCs commitment. Therefore, our data collectively identified myb-driven hsa-miR-486-3p upregulation and subsequent c-maf downregulation as a new molecular mechanism through which cMyb favours erythropoiesis while restraining megakaryopoiesis.
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| Overall design |
RNA from CD34+ HPCs transfected once/twice/3 times with c-myb-targeting/non targeting control siRNAs was collected for a set of 5 independent experiments.
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| Contributor(s) |
Bianchi E, Bulgarelli J, Sacchi G, Ruberti S, Norfo R, Rontauroli S, Pennucci V, Zini R, Salati S, Prudente Z, Ferrari S, Manfredini R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 11, 2014 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Rossella Manfredini |
| E-mail(s) |
manfredini.rossella@unimore.it
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| Phone |
+390592058065
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| Organization name |
Centre for Regenerative Medicine
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| Department |
Life Sciences
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| Street address |
Via Gottardi 100
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| City |
Modena |
| ZIP/Postal code |
41100 |
| Country |
Italy |
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| Platforms (1) |
| GPL13667 |
[HG-U219] Affymetrix Human Genome U219 Array |
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| Samples (30)
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| GSM1470591 |
NegCTR CD34+ HPCs, 1 Nucleofection, donor 1 |
| GSM1470592 |
myb-silenced CD34+ HPCs, 1 Nucleofection, donor 1 |
| GSM1470593 |
NegCTR CD34+ HPCs, 2 Nucleofections, donor 1 |
| GSM1470594 |
myb-silenced CD34+ HPCs, 2 Nucleofections, donor 1 |
| GSM1470595 |
NegCTR CD34+ HPCs, 3 Nucleofections, donor 1 |
| GSM1470596 |
myb-silenced CD34+ HPCs, 3 Nucleofections, donor 1 |
| GSM1470597 |
NegCTR CD34+ HPCs, 1 Nucleofection, donor 2 |
| GSM1470598 |
myb-silenced CD34+ HPCs, 1 Nucleofection, donor 2 |
| GSM1470599 |
NegCTR CD34+ HPCs, 2 Nucleofections, donor 2 |
| GSM1470600 |
myb-silenced CD34+ HPCs, 2 Nucleofections, donor 2 |
| GSM1470601 |
NegCTR CD34+ HPCs, 3 Nucleofections, donor 2 |
| GSM1470602 |
myb-silenced CD34+ HPCs, 3 Nucleofections, donor 2 |
| GSM1470603 |
NegCTR CD34+ HPCs, 1 Nucleofection, donor 3 |
| GSM1470604 |
myb-silenced CD34+ HPCs, 1 Nucleofection, donor 3 |
| GSM1470605 |
NegCTR CD34+ HPCs, 2 Nucleofections, donor 3 |
| GSM1470606 |
myb-silenced CD34+ HPCs, 2 Nucleofections, donor 3 |
| GSM1470607 |
NegCTR CD34+ HPCs, 3 Nucleofections, donor 3 |
| GSM1470608 |
myb-silenced CD34+ HPCs, 3 Nucleofections, donor 3 |
| GSM1470609 |
NegCTR CD34+ HPCs, 1 Nucleofection, donor 4 |
| GSM1470610 |
myb-silenced CD34+ HPCs, 1 Nucleofection, donor 4 |
| GSM1470611 |
NegCTR CD34+ HPCs, 2 Nucleofections, donor 4 |
| GSM1470612 |
myb-silenced CD34+ HPCs, 2 Nucleofections, donor 4 |
| GSM1470613 |
NegCTR CD34+ HPCs, 3 Nucleofections, donor 4 |
| GSM1470614 |
myb-silenced CD34+ HPCs, 3 Nucleofections, donor 4 |
| GSM1470615 |
NegCTR CD34+ HPCs, 1 Nucleofection, donor 5 |
| GSM1470616 |
myb-silenced CD34+ HPCs, 1 Nucleofection, donor 5 |
| GSM1470617 |
NegCTR CD34+ HPCs, 2 Nucleofections, donor 5 |
| GSM1470618 |
myb-silenced CD34+ HPCs, 2 Nucleofections, donor 5 |
| GSM1470619 |
NegCTR CD34+ HPCs, 3 Nucleofections, donor 5 |
| GSM1470620 |
myb-silenced CD34+ HPCs, 3 Nucleofections, donor 5 |
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| This SubSeries is part of SuperSeries: |
| GSE60301 |
MYB/miR4863p/MAF/ axis in hematopoietic progenitor cell fate decision |
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| Relations |
| BioProject |
PRJNA257985 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE60299_RAW.tar |
62.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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