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| Status |
Public on Jul 14, 2015 |
| Title |
Real-time quantitative PCR analysis for validation of miRNAs expressed during osteogenic differentiation of bone marrow MSCs versus healthy donors MSCs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by RT-PCR
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| Summary |
Total small RNA was isolated from BM-MSCs obtained from OA diagnosed patients (n=10) and non-OA (n=10 after osteogenic induction for 1, 10 and 21 days. miRNAs, were initially screened and those with consistent differential expression were selected for further validation by qRT-PCR analysis.
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| Overall design |
From an initial screening of 246 differentially expressed miRNAs (fold change ≥± 2, P ≤0.05) between OA and non-OA BM-MSC samples 21 were selected and validated by qRT-PCR
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| Contributor(s) |
Tornero-Esteban P, Rodríguez-Rodríguez L, Abásolo L, Tomé M, López-Romero P, Herranz E, González MA, Marco F, Moro E, Fernández-Gutiérrez B, Lamas J |
| Citation(s) |
26243143 |
| Submission date |
Jul 13, 2015 |
| Last update date |
Oct 21, 2015 |
| Contact name |
José Ramón Lamas Lamas |
| E-mail(s) |
jrlamas@gmail.com
|
| Phone |
+34913303615
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| Organization name |
Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC)
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| Department |
Rheumatology
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| Lab |
Medicina y Cirugía Experimental. Laboratorio 2
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| Street address |
Calle Profesor Martín Lagos s/n. Hospital Clínico san Carlos
|
| City |
Madrid |
| State/province |
MADRID |
| ZIP/Postal code |
28220 |
| Country |
Spain |
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| Platforms (1) |
| GPL20685 |
Exiqon Human miRCURY LNA RT miRNA PCR |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA289693 |
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