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Sample GSM18728 Query DataSets for GSM18728
Status Public on Mar 19, 2004
Title 1BJZ02022102_leukemialymphoblastic(molt4)
Sample type RNA
 
Source name 1BJZ02022102_leukemialymphoblastic(molt4)
Organism Homo sapiens
Characteristics 1BJZ02022102_leukemialymphoblastic(molt4)
Extracted molecule total RNA
Extraction protocol Human tissue samples were obtained from several sources: Clinomics Biosciences (Pittsfield, MA), Clontech, AllCells (Berkeley, CA), Clonetics/BioWhittaker (Walk-ersville, MD), AMS Biotechnology (Abingdon, Oxfordshire, U.K.), and the University of California at San Diego. When samples from four or more subjects were available, equal numbers of male and female subjects were used to make two independent pools; when fewer than four samples were available, RNA samples were pooled, and duplicate amplifications were performed for each pool (detailed annotation for human samples is on our web site, http:\\symatlas.gnf.org) Extracted RNA was additionally purified on RNeasy mini columns (Qiagen). RNA quantity and quality was verified on Bioanalyser 2100 (Agilent).
Label biotin
Label protocol Five micrograms of total RNA was used to synthesize cDNA that was then used as a template to generate biotinylated cRNA using Enzo kits.
 
Hybridization protocol standard Affymetrix procedures except for use of a custom 24-channel fluidics machine and GCS2500 scanner
Scan protocol standard Affymetrix procedures
Description leukemialymphoblastic(molt4)
Data processing MAS5
 
Submission date Mar 18, 2004
Last update date Sep 25, 2006
Contact name John R Walker
E-mail(s) jwalker@gnf.org
Phone 858-812-1636
Organization name Genomics Institute of the Novartis Research Foundation
Lab Genetics Core
Street address 10675 John Jay Hopkins
City San Diego
State/province CA
ZIP/Postal code 92121
Country USA
 
Platform ID GPL1074
Series (1)
GSE1133 tissue-specific pattern of mRNA expression

Data table header descriptions
ID_REF
VALUE 'signal' a measure of the abundance of a transcript

Data table
ID_REF VALUE
gnf1h00001_x_at 23.8
gnf1h00002_x_at 58.2
gnf1h00003_x_at 27.2
gnf1h00004_x_at 25.8
gnf1h00005_x_at 35.1
gnf1h00006_x_at 8.5
gnf1h00007_x_at 19.4
gnf1h00008_x_at 33.4
gnf1h00009_x_at 14.8
gnf1h00010_x_at 34.3
gnf1h00011_x_at 14.8
gnf1h00012_x_at 12.4
gnf1h00013_x_at 31.8
gnf1h00014_x_at 11.4
gnf1h00015_x_at 104.8
gnf1h00031_at 10.7
gnf1h00032_at 14.5
gnf1h00033_at 76.8
gnf1h00034_s_at 69.7
gnf1h00035_at 182.9

Total number of rows: 11391

Table truncated, full table size 216 Kbytes.




Supplementary file Size Download File type/resource
GSM18728.CEL.gz 3.5 Mb (ftp)(http) CEL

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