|
| Status |
Public on Feb 15, 2011 |
| Title |
leat-S_12H_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
2 week-old wheat leaves 12 hours after powdery mildew infection
|
| Organism |
Triticum aestivum |
| Characteristics |
line: 8866 (susceptible to wheat powdery mildew,also designed as ‘S’)
age: two-week old
tissue: leaf
infection: powdery mildew
time: 12 hours
|
| Treatment protocol |
One isolate of the powdery mildew fungus Erysiphe graminis f. sp. tritici (Egt) (Isolate E09) was maintained on the susceptible wheat cultivar by weekly transfer to new plants. Inoculations were performed at a density of 100-150 conidia/mm2 by brushing plants. Leaves were collected at 12 hours after infection.
|
| Growth protocol |
Hexaploid wheat (Triticum aestivum L.) line 8866 (S)(susceptible to wheat powdery mildew) and its near-isogenic resistant line Pm30 (R) were grown in a growth chamber at a relative humidity of 75% and 26/20℃ day and night temperature with light intensity of 3000 lx. 2 week-old plants were used for all experiments.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The biotin labelled cRNA was made using the GeneChip® IVT Labelling Kit (Affymetrix, CA, USA).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on GeneChip wheat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Genechip® 3000 Scanner
|
| Description |
2-8866-12H
|
| Data processing |
The chip images were scanned and extracted using default settings and the CEL files were produced with the Affymetrix GeneChip Operating Software (GCOS 1.2). The resulting CEL files were imported into the Bioconductor (version 1.8) in R 2.3.0 statistical environment. Subsequent background adjustment, normalization of the raw data and estimation of probe sets signal intensities were done using GC-RMA method.
|
| |
|
| Submission date |
Feb 15, 2011 |
| Last update date |
Feb 15, 2011 |
| Contact name |
mingming xin |
| E-mail(s) |
xmmlyac@126.com
|
| Phone |
86-010-62731353
|
| Organization name |
China Agricultrual University
|
| Department |
plant genetics and breeding
|
| Lab |
wheat genetics and genomics
|
| Street address |
YUAN MING YUAN WEST ROAD 2, HAIDIAN DISTRICT
|
| City |
BEIJING |
| ZIP/Postal code |
100193 |
| Country |
China |
| |
|
| Platform ID |
GPL3802 |
| Series (2) |
| GSE27320 |
Expression data in wheat (T. aestivum L.) near isogenic lines in response to powdery mildew infection |
| GSE27339 |
Wheat response to powdery mildew infection and heat stress |
|
