|
Status |
Public on Feb 16, 2015 |
Title |
Harvard Sib female CHB1000005950 |
Sample type |
genomic |
|
|
Source name |
leukocyte
|
Organism |
Homo sapiens |
Characteristics |
group: Harvard.Sib.female gender: F sentrix id: 5775446055 sentrix position: R04C02 experiment: 2 age at collection months: 58 ethnicity: Black cell type: leukocyte
|
Treatment protocol |
Extracted DNA was bisulfite treated with the Zymo bisulfite kit according the manufacturer's specifications
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was extracted by the Rutgers University Cell and DNA Repository RUCDR
|
Label |
biotin nucleotide or dinitrophenyl labeled
|
Label protocol |
A single nucleotide extension was labeled either with biotin or dinitrophenyl according to the manufacturer's (Illumina) specifications
|
|
|
Hybridization protocol |
Followed the manufacturer's (Illumina) specifications
|
Scan protocol |
Followed the manufacturer's (Illumina) specifications on either a BeadStation 500 or an iScan
|
Description |
Harvard.Sib.female
|
Data processing |
Avg beta (β), detection value, and signal for Probe A (unmethylated) and Probe B were exported from GenomeStudio v2010.3 Methylation Module 1.8.5. The manufacturer measures methylation through the beta (β) value calculated as probe B signal / (probe A signal + probe B signal + 100). Based on better reproducibility of the controls we calculated the methylation using a metric denoted beta prime (β') which is calculated as probe B signal / (probe A signal + probe B signal).
|
|
|
Submission date |
Feb 23, 2012 |
Last update date |
Feb 16, 2015 |
Contact name |
Benjamin G Barwick |
E-mail(s) |
benjamin.barwick@emory.edu
|
Phone |
(404) 285-2964
|
Organization name |
Emory University
|
Department |
Hematology and Medical Oncology
|
Lab |
Barwick Lab
|
Street address |
1365 Clifton Rd. NE WCI-C 4th Floor Benches 36-37
|
City |
Atlanta |
State/province |
GA |
ZIP/Postal code |
30322 |
Country |
USA |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE36054 |
Methylation Profiling of Blood DNA from Healthy Children |
|