|
| Status |
Public on Mar 17, 2014 |
| Title |
LTED YC-1 |
| Sample type |
RNA |
| |
|
| Source name |
LTED cell line treated
|
| Organism |
Homo sapiens |
| Characteristics |
cell line source: Breast cancer
agent: YC-1 (2 micrograms/L)
cell line: LTED
|
| Treatment protocol |
MCF7 cells were treated with YC-1 (20 micrograms/L) and LTED cells were treated with YC-1 (2 micrograms/L) or vehicle (DMSO) for 48 hours.
|
| Growth protocol |
MCF7 cells were cultured and maintained in RPMI medium containing 10% FBS and 2 mM glutamine and LTED cells were cultured and maintained in RPMI medium containing 10% DCC-FBS (estrogen depleted) and 2 mM glutamine.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
FL-Ovation cDNA Biotin Module V2 from NuGen Technologies Inc.
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev 2).
|
| Scan protocol |
Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev 2).
|
| Data processing |
The data were analyzed with Partek Genomics Suite using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jun 20, 2012 |
| Last update date |
Mar 17, 2014 |
| Contact name |
Miguel Angel Pujana |
| E-mail(s) |
mapujana@iconcologia.net
|
| Phone |
0034932607463
|
| Organization name |
Institut Catala d'Oncologia IDIBELL
|
| Lab |
ProCURE
|
| Street address |
Gran Via 199
|
| City |
Barcelona |
| ZIP/Postal code |
08908 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE38829 |
Expression data from MCF7 and MCF7-LTED cells treated with YC-1 |
|
