1M2V


Conserved Protein Domain Family
Sec24-like

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cd01479: Sec24-like 
Click on image for an interactive view with Cn3D
Sec24-like: Protein and membrane traffic in eukaryotes is mediated by at least in part by the budding and fusion of intracellular transport vesicles that selectively carry cargo proteins and lipids from donor to acceptor organelles. The two main classes of vesicular carriers within the endocytic and the biosynthetic pathways are COP- and clathrin-coated vesicles. Formation of COPII vesicles requires the ordered assembly of the coat built from several cytosolic components GTPase Sar1, complexes of Sec23-Sec24 and Sec13-Sec31. The process is initiated by the conversion of GDP to GTP by the GTPase Sar1 which then recruits the heterodimeric complex of Sec23 and Sec24. This heterodimeric complex generates the pre-budding complex. The final step leading to membrane deformation and budding of COPII-coated vesicles is carried by the heterodimeric complex Sec13-Sec31. The members of this CD belong to the Sec23-like family. Sec 24 is very similar to Sec23. The Sec23 and Sec24 polypeptides fold into five distinct domains: a beta-barrel, a zinc finger, a vWA or trunk, an all helical region and a carboxy Gelsolin domain. The members of this subgroup carry a partial MIDAS motif and have the overall Para-Rossmann type fold that is characteristic of this superfamily.
Statistics
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PSSM-Id: 238756
Aligned: 15 rows
Threshold Bit Score: 300.729
Created: 10-Jan-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 2 residues -Click on image for an interactive view with Cn3D
Feature 1:partial metal ion-dependent adhesion site (MIDAS)
Evidence:
  • Comment:binding of divalent ions is mediated by the MIDAS motif

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                # #                                                                   
1M2V_B     301 PPPATYCFLIDVSQSSIKSGLLATTINTLLQNLDSIPNHd----ERTRISILCVDNAIHYFKIPLdsennee-------- 368  baker's yeast
1730731    234 PPPSVYVFLLDVSQNAVKNGLLATSARTILENIEFLPNHd----GRTRIAIICVDHSLHYFYVPLdddyevsdeddeesd 309  baker's yeast
5734485    327 PQPLVYVFLIDISFVSISSGMVGTASRAILESLDRIPNKe----GRAKVAFIGVDSALHFFSVSPgae------------ 390  fission yeast
25005158   403 PQPAVYVFVIDISAAAIQSGYLHTFAEQLLINIDQMPGD-----DRSQVCFIAVDQCLHFFSFSSnkr------------ 465  Caenorhabditis e...
13124520   502 PMPAVYFFLIDVSMNAIQTGATAAACNAIQQVLSDLPEG-----PRTFVGIATFDSTIHFYNLKRalq------------ 564  thale cress
13124526   441 PMPPIYFFLIDVSISATKSGMLEVVAQTIKSCLDNLPGY-----PRTQIGFITYDSTLHFYNMKSsls------------ 503  thale cress
13124508   437 PNPPAFIFMIDVSYSNIKNGLVKLICEELKTMLEKIPKEeqeetSAIRVGFITYNKVLHFFNVKSnla------------ 504  human
27735256   499 PSPPAFIFMIDVSYNAIRTGLVRLLCEELKSLLDFLPREggaeeSAIRVGFVTYNKVLHFYNVKSsla------------ 566  human
22945393   599 PEVPAFIFIIDVSYNTVKSGLVHLLCSQIKNILKHLPVDqgqdkSKVRVGFITYNSTVHFYNIKSsla------------ 666  fruit fly
3875796    531 PKEPAFIFMIDVSYNAISNGMLPILCQNLEKVLRNLPREsgqleSSMRIGLATFDQAVHFFDISSas------------- 597  Caenorhabditis e...
Feature 1                                                                                      
1M2V_B     369 --------------sadqinMMDIADLEEPFLPRPNSMVVSLKACRQNIETLLTKI-PQIFQSNLIT--NFALGPALKSA 431  baker's yeast
1730731    310 geeededeeeedvdnsetiqMFDIGDLDEPFLPMPSDELVVPLKYCKNNLETLLKKiPEIFQDTHSS--KFALGPALKAA 387  baker's yeast
5734485    391 -----------------eatQLVVSDLEEPFLPRNQDLLLNLRECRQGIENLLERF-QSMFATTRDS--SNALGPGLKAA 450  fission yeast
25005158   466 -----------------ypnEMIVDDIDDAFLPSVTSLLVPMKKFKDTIRTFIKQL-PEFYSQIGPTsnGNCLGSALKLA 527  Caenorhabditis e...
13124520   565 -----------------qplMLIVPDVQDVYTPLETDVVVQLSECRQHLELLLDSI-PTMFQESKIP--ESAFGAAVKAA 624  thale cress
13124526   504 -----------------qpqMMVVSDLDDIFVPLPDDLLVNLSESRTVVDAFLDSL-PLMFQDNFNV--ESAFGPALRAA 563  thale cress
13124508   505 -----------------qpqMMVVTDVGEVFVPLLDGFLVNYQESQSVIHNLLDQI-PDMFADSNEN--ETVFASVIQAG 564  human
27735256   567 -----------------qpqMMVVSDVADMFVPLLDGFLVNVNESRAVITSLLDQI-PEMFADTRET--ETVFVPVIQAG 626  human
22945393   667 -----------------qpqMMVVGDVQEMFMPLLDGFLCHPEESAAVIDALMEEI-PRMFADTKET--ETILYPAIQAG 726  fruit fly
3875796    598 ------------------pkMLVMSDVQEPFVPMVDGLLLPYNEALPGLRAALAEI-PKLFSQSKTT--ETILQPVVQAG 656  Caenorhabditis e...
Feature 1                                                                                      
1M2V_B     432 YHLIG--GVGGKIIVVSGTLPNLGI-GKLQRRNE------SGVVNtsketaqLLSCQDSFYKNFTIDCSKVQITVDLFLA 502  baker's yeast
1730731    388 SNLIK--STGGKVEVISSTLPNTGI-GKLKKRSEqg--ilNTPKEss----qLLSCKDSFYKTFTIECNKLQITVDMFLA 458  baker's yeast
5734485    451 HRLIE--NIGGKICCLISSLPNVGV-GKLELREDpk--llGTNREs-----sLLHPNDSFYKSFAVECSTSQVSVDMFLF 520  fission yeast
25005158   528 QSMIQ--EVGGRISIFQVSLPNLGL-GALKSREE------STEGGq------NLGPATDFYKALSLECTGSQICLDLFMF 592  Caenorhabditis e...
13124520   625 FLAMK--SKGGKLMVFQSILCSVGV-GALSSREAegranmSAGEKea---hkLLQPADKTLKTMAIEFAEYQVCVDIFIT 698  thale cress
13124526   564 FMVMN--QLGGKLLIFQNSLPSLGA-GRLKLRGDdpr-vyGTDKEy-----aLRVAEDPFYKQMAADCTKFQIGINVYAF 634  thale cress
13124508   565 MEALKaaDCPGKLFIFHSSLPTAEApGKLKNRDDkk--lvNTDKEk-----iLFQPQTNVYDSLAKDCVAHGCSVTLFLF 637  human
27735256   627 MEALKaaECAGKLFLFHTSLPIAEApGKLKNRDDrk--liNTDKEk-----tLFQPQTGAYQTLAKECVAQGCCVDLFLF 699  human
22945393   727 LEALKasNAAGKLLVFNSTLPIAEApGKLKNRDDrk--llGTDKEk-----tVLTPQTTAYNTLGQECVQQGCSVDLFVF 799  fruit fly
3875796    657 LDALKcaDRAGKVILFSTVLPTYDApGKLKSKNDrs--llGTDKEk-----nALVPQEDSYTKLGEQCVKSGVTVDLFLF 729  Caenorhabditis e...
Feature 1                                                                 
1M2V_B     503 SEDYMDVASLSNLSRFTAGQTHFYpg---------fsGKNPNDIVKFSTEFAKHISMDF 552  baker's yeast
1730731    459 SEDYMDVATLSHLGRFSGGQTHFYpg---------fnATSLNDVTKFTRELSRHLSMDI 508  baker's yeast
5734485    521 SSQYQDVATLSCLPRYTSGKTQFYhr---------wnASRSEDALKFASELTNYLSMEI 570  fission yeast
25005158   593 NTQYADLATLSEIAKFSTGCVYHFpny--------nyRNDIHQVKRFERTLTRYLTRKL 643  Caenorhabditis elegans
13124520   699 TQAYVDMASISVIPRTTGGQVYCYy-----------pFSALSDPPKLYNDLKWNITRPQ 746  thale cress
13124526   635 SDKYTDIASLGTLAKYTGGQVYYYp-----------gFQSSVHGDKLRHELARDLTRET 682  thale cress
13124508   638 PSQYVDVASLGLVPQLTGGTLYKYnnfqmhldrqqflNDLRNDIEKKIGFDAIMRVRTS 696  human
27735256   700 PNQYVDVATLSVVPQLTGGSVYKYasfqvendqerflSDLRRDVQKVVGFDAVMRVRTS 758  human
22945393   800 NNAYIDLATIGQVSRLTGGEVFKYtyfqa----dvdgKRLIQDIIKNVSRPIAFDAVMR 854  fruit fly
3875796    730 PNAFIDVATIGQLSAVTGGSIFKFq-----------yFSADKDGVRMLNELERHVSKKI 777  Caenorhabditis elegans

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