GEO DataSets

(Submitter supplied) Background: Tissue kallikrein-related peptidases 4, 5, 6 and 7 (KLK4-7) strongly increase the malignancy of ovarian cancer cells. By further deciphering the downstream effectors of KLK4-7, we aimed at finding new potential prognostic biomarkers and treatment targets for ovarian cancer patients. KLK4-7-transfected and vector-control OVMZ-6 (OV-KLK4-7 and OV-VC) ovarian cancer cells were established to select differentially regulated factors. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16604

6 Samples

Download data: TXT, XLSX

(Submitter supplied) The propensity of cancer cells to transition between epithelial and mesenchymal phenotypic states is considered to be critical in metastatic processes, cancer progression, and treatment resistance. To investigate a transient epithelial-mesenchymal transition (EMT) in prostate adenocarcinoma, LNCaP cells with inducible and reversible expression of SNAI1 or SNAI2 were generated. Cells were treated with doxycycline to induce a SNAI1- or SNAI2-mediated EMT for 5 days and then subsequently removed for 3, 5, and 20 days to allow for MET. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16604

44 Samples

Download data: TXT

(Submitter supplied) Using tissue engineering technologies, we aimed to engineer the ovarian tumor microenvironment to study disease progression by combining biomimetic hydrogels with melt electrospun written scaffolds. 3D co-cultures were constructed by assembling ovarian cancer cell-laden hydrogels with mesothelial cell-layered scaffolds. 3D constructs were characterised by proliferation and transcriptomic analyses and applied to an intraperitoneal xenograft model. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16604

12 Samples

Download data: TXT, XLSX

(Submitter supplied) Bioassay-guided fractionation of a cytotoxic extract derived from the Australian endemic plant Hernandia albiflora (twigs) resulted in the isolation of the two known compounds: deoxypodophyllotoxin and thalicthuberine. Both compounds were tested for their inhibition of LNCaP cell viability. Thalicthuberine (TH, IC50=2.0 µM) was selected for mechanism of action studies. Ongoing investigations have shown that TH induced a strong accumulation of LNCaP cells in mitosis, severe mitotic spindle defects and asymmetric cell divisions, ultimately leading to mitotic catastrophe accompanied by cell death through apoptosis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16604

9 Samples

Download data: TXT

(Submitter supplied) The lack of a cure for metastatic prostate cancer (PCa) highlights the urgent need for more efficient drugs to fight this disease. Here, we report the molecular mechanism of action of the natural product 6-acetoxy-anopterine (6-AA) in malignant cells of the prostate. This potent cytotoxic alkaloid from the endemic Australian tree Anopterus macleayanus induced at low nanomolar doses a strong accumulation of LNCaP and PC3 PCa cells in mitosis, severe mitotic spindle defects and asymmetric cell divisions, ultimately leading to mitotic catastrophe accompanied by cell death through apoptosis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16604

10 Samples

Download data: TXT

(Submitter supplied) As part of an anti-cancer natural product drug discovery program, we recently identified eusynstyelamide B (EB), which displayed cytotoxicity against MDA-MB-231 breast cancer cells. Here, we investigated the mechanism of action of EB in the prostate cancer cell line LNCaP after a treatment with 5 uM for 24 h. Transcriptome profiling followed by pathway analysis suggested that EB activated DNA damage pathways in LNCaP cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16604

9 Samples

Download data: TXT

(Submitter supplied) Epigenetic silencing mediated by CpG methylation is a common feature of many cancers. Characterizing aberrant DNA methylation changes associated with tumor progression may identify potential prognostic markers for prostate cancer (PCa). We treated three PCa cell lines, 22Rv1, DU-145 and LNCap with the demethylating agent 5-aza 2’–deoxycitidine (DAC) and examined gene expression changes using a custom microarray (GPL16604). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16604

18 Samples

Download data: TXT

(Submitter supplied) Knockdown of the receptor tyrosine kinase EphB4 using siRNA in LNCaP prostate cancer cells compared to negative siRNA controls

Organism:

Homo sapiens

Type:

Expression profiling by genome tiling array

Platform:

GPL16604

7 Samples

Download data: TXT

(Submitter supplied) Analysis of LNCaP cell molecular differences in monocultures and in co-cultures in the presence of R1881. Human osteoblast molecular signatures were also identified from the tissue engineered bone monocultures. LNCaP cells molecular profile was altered by co-culturing with human osteoblasts compared to LNCaP monocultures with or without R1881 stimulations. These results provide insights into the behavioral change of LNCaP cells in a bone-like microenvironment.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16604

24 Samples

Download data: TXT

(Submitter supplied) The androgen receptor (AR) plays a central role in the development of castration resistant prostate cancer (CRPC). Here, we demonstrate that the ubiquitin ligase Siah2 targets a select pool of NCOR1-bound, transcriptionally inactive AR for ubiquitination dependent degradation, thereby promoting the expression of ~13% of AR target genes. The Siah2 binding sites located within the AR ligand-binding domain are mutated in PCa, resulting in attenuation of Siah2-mediated regulation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16604

52 Samples

Download data: TXT