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Items: 5

1.

An automatec microwell platform for large-scale single cell RNA-seq.

(Submitter supplied) We report an automated microwell array platform for single cell RNA-seq with significantly improved performance over previous implementations. We demonstrate cell capture efficiencies of >50%, compatibility with commercially available barcoded mRNA capture beads, and parallel expression profiling from thousands of individual cells. We apply our system to comprehensively assess heterogeneity in gene expression of patient-derived glioma neurospheres and uncover subpopulations similar to those observed in human glioma tissue.
Organism:
Mus musculus; Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL19415 GPL18573
2 Samples
Download data: TXT
Series
Accession:
GSE85575
ID:
200085575
2.

Illumina NextSeq 500 (Homo sapiens; Mus musculus)

Organism:
Homo sapiens; Mus musculus
93 Series
1635 Samples
Download data
Platform
Accession:
GPL19415
ID:
100019415
3.

Illumina NextSeq 500 (Homo sapiens)

Platform
Accession:
GPL18573
ID:
100018573
4.

U87_3T3_scRNAseq

Organism:
Homo sapiens; Mus musculus
Source name:
Brain and embyro
Platform:
GPL19415
Series:
GSE85575
Download data: TXT
Sample
Accession:
GSM2277952
ID:
302277952
5.

TS543_scRNAseq

Organism:
Homo sapiens
Source name:
Brain
Platform:
GPL18573
Series:
GSE85575
Download data: TXT
Sample
Accession:
GSM2277951
ID:
302277951
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Supplemental Content

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