GEO DataSets

(Submitter supplied) The method of mRNA tagging was recently developed to isolate mRNA population from muscle tissues in Caenohabditis elegans. This method utilizes a FLAG-tagged poly(A)-binding protein (PABP), which can bind to poly(A)-tail of eukaryotic mRNA. Thus, the mRNA population from specific tissue, in which FLAG- tagged PABP is expressed, can be co-immunoprecipitated with FLAG-tagged PABP using FLAG-specific antibody. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Dataset:

GDS2479

Platform:

GPL72

14 Samples

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Analysis of mRNA purified by mRNA-tagging from photoreceptors cells. These cells are engineered to express a FLAG-tagged poly(A)-binding protein (PABP) that allows separation of their mRNA from those of cells not expressing the tagged PABP. Results provide insight into the validity of this approach.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, count, 2 genotype/variation, 2 tissue sets

Platform:

GPL72

Series:

GSE1790

14 Samples

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(Submitter supplied) Affymetrix submissions are typically submitted to GEO using the GEOarchive method described at http://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/projects/geo/info/geo_affy.html Has 14010 entries and was indexed 29-Jan-2002 The sequences used to develop this array are accessible through FlyBase. Probes were synthesized in situ. Contains >13,500 gene sequences predicted from annotation of the Drosophila genome version 1, >8000 sequences for which confirming EST or full-length cDNA evidence is available, ~3,000 genes identified using prediction algorithms, and ~2,500 genes that not only have a gene predicted algorithmically, but also show some homology to genes from other organisms. more...

Organism:

Drosophila melanogaster

37 DataSets

111 Series

1233 Samples

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