GEO DataSets

(Submitter supplied) Skin and bladder epithelia form effective permeability barriers through the activation of distinct differentiation gene programs. Employing a genome-wide gene expression study, we identified transcription regulators whose expression correlates highly with that of differentiation markers both in bladder and skin, including the Grainyhead factor Get1/Grhl3, already known to be important for epidermal barrier formation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL, CHP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

19 Samples

Download data: CEL, CHP

(Submitter supplied) Skin and bladder epithelia form effective permeability barriers through the activation of distinct differentiation gene programs. Employing a genome-wide gene expression study, we identified transcription regulators whose expression correlates highly with that of differentiation markers both in bladder and skin, including the Grainyhead factor Get1/Grhl3, already known to be important for epidermal barrier formation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

8 Samples

Download data: CEL, CHP

(Submitter supplied) Skin and bladder epithelia form effective permeability barriers through the activation of distinct differentiation gene programs. Employing a genome-wide gene expression study, we identified transcription regulators whose expression correlates highly with that of differentiation markers both in bladder and skin, including the Grainyhead factor Get1/Grhl3, already known to be important for epidermal barrier formation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

8 Samples

Download data: CEL, CHP

(Submitter supplied) Defective permeability barrier is an important feature of many skin diseases and causes mortality in premature infants. To investigate the control of barrier formation, we characterized the epidermally expressed Grainyhead-like epithelial transactivator (Get-1)/Grhl3, a conserved mammalian homologue of Grainyhead, which plays important roles in cuticle development in Drosophila. Get-1 interacts with the LIM-only protein LMO4, which is co-expressed in the developing mammalian epidermis. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2629

Platform:

GPL1261

6 Samples

Download data: CEL, CHP

Analysis of skins of embryos lacking Grainyhead-like epithelial transactivator (Get-1). Results provide insight into the role of Get-1 in epidermal differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE7381

6 Samples

Download data: CEL, CHP

(Submitter supplied) Whether epidermal factors play a primary role in immune-mediated skin diseases such as psoriasis is unknown. We now show that the pro-differentiation transcription factor Grainyhead-like 3 (GRHL3), essential during epidermal development but dispensable in adult skin homeostasis, is required for barrier repair after adult epidermal injury. Consistent with activation of a GRHL3-regulated repair pathway in psoriasis, we find GRHL3 up-regulation in lesional skin where GRHL3 binds known epidermal differentiation gene targets. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

20 Samples

Download data: CEL

(Submitter supplied) Epidermal barrier repair mechanisms activated in psoriasis lesions are likely involved in limiting the severity of this disease. We show that loss of grainyhead-like 3 (Grhl3), a pro-terminal differentiation factor in the epidermis, is sufficient to trigger greater sensitivity to and delayed resolution of epidermal lesions resulting from either physical or immune mediated barrier injury. After stimulation of Toll like receptors, the loss of Grhl3 resulted in increased epidermal damage with a striking increase in basal cell proliferation, hyperplasia of partially differentiated suprabasal layers, and a transcriptional profile highlighted by the overexpression of epidermal wound response and alarmin genes. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13112

6 Samples

Download data: BED, TXT

(Submitter supplied) Objectives: Much of the information to date in terms of subtypes and function of bladder urothelial cells were derived from anatomical location or by the expression of a small number of marker genes. To have a comprehensive map of the cellular anatomy of bladder urothelial cells, we performed single-cell RNA-sequencing to thoroughly characterize mouse bladder urothelium. Materials and methods: A total of 18,917 single cells from mouse bladder urothelium was analyzed by unbiased single-cell RNA sequencing. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

1 Sample

Download data: MTX, TSV

(Submitter supplied) We developed a method for the directed differentiation of hiPSCs into mature stratified bladder urothelium.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL15207

1 Sample

Download data: CEL

(Submitter supplied) Kruppel-like transcription factor 5 (Klf5) is expressed during late embryogenesis in the forming murine bladder urothelium. Targeted disruption of the Klf5flox alleles by the ShhGfpCre transgene resulted in failure of the bladder urothelium to mature accompanied by hydronephrosis, hydroureter, and vesicoureteric reflux in all E18.5 fetuses. The bladder urothelium did not stratify nor did it express terminal differentiation markers characteristic of basal, intermediate, and umbrella cells including keratins 20, 14, and 5, and uroplakins. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5200

Platform:

GPL6246

6 Samples

Download data: CEL

Analysis of fetal bladders with Kruppel-like transcription factor 5 (KLF5) deficiency specific to bladder epithelial cells. KLF5 is expressed in proliferative epithelial cell types during embryogenesis. Results provide insight into the role of KLF5 in the development of the bladder urothelium.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 genotype/variation sets

Platform:

GPL6246

Series:

GSE27014

6 Samples

Download data: CEL

(Submitter supplied) The antagonistic actions of Polycomb and Trithorax are responsible for proper cell fate determination in mammalian tissues. In the epidermis, a self-renewing epithelium, previous work has shown that release from Polycomb repression only partially explains differentiation gene activation. We now show that Trithorax is also a key regulator of epidermal differentiation, not only through activation of genes repressed by Polycomb in progenitor cells, but also through activation of genes independent of regulation by Polycomb. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL11154

3 Samples

Download data: TXT

(Submitter supplied) Human primary keratinocytes were collected at 0, 1, 3, 6, 12, 24 and 48 hours after addition of 1.8mM Calcium and RNA was extracted.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5454

Platform:

GPL6244

14 Samples

Download data: CEL, CHP

(Submitter supplied) Human primary keratinocytes were depleted of MLL2 by siRNA and induced to differentiated for 2 days by addition of Calcium

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5452

Platform:

GPL6244

4 Samples

Download data: CEL, CHP

(Submitter supplied) Human primary keratinocytes were depleted of GRHL3 by siRNA and induced to differentiated for 2 days by addition of Calcium

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5453

Platform:

GPL6244

6 Samples

Download data: CEL, CHP

Analysis of neonatal epidermal keratinocytes treated with 1.8 mM calcium for up to 48 hours. Results provide insight into the molecular mechanisms underlying the differentiation of epidermal keratinocytes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 7 time sets

Platform:

GPL6244

Series:

GSE38628

14 Samples

Download data: CEL, CHP

Analysis of Grainyhead like 3 (GRHL3)-depleted neonatal epidermal keratinocytes following treatment with 1.8 mM calcium for 24 hours to induce differentiation. GRHL3 encodes a transcription factor. Results provide insight into the role of GRHL3 in keratinocyte differentiation.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL6244

Series:

GSE37049

6 Samples

Download data: CEL, CHP

Analysis of MLL2-depleted neonatal epidermal keratinocytes following treatment with 1.8 mM calcium for 24 hours to induce differentiation. Histone H3K4 methyltransferase MLL2 is a trithorax group protein. Results provide insight into the role of MLL2 in keratinocyte differentiation.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL6244

Series:

GSE37570

4 Samples

Download data: CEL, CHP

(Submitter supplied) Peroxisome Proliferator-Activated Receptor-gamma (PPARG) is a nuclear hormone receptor that was originally described as a master regulator of adipogenesis but could also promote cellular differentiation in a number of epithelium. PPARG also serves as an important regulator in anti-inflammatory activity after a variety of injuries, acting in part by antagonizing the NF-kB pathway. Moreover, the expression of PPARG is strongly down regulated in the basal subtype of bladder cancer, suggesting that its removal might be essential in tumorigenesis. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

26 Samples

Download data: TXT