GEO DataSets

(Submitter supplied) We showed that nandrolone attenuated subacute, but not acute, denervation atrophy and upregulation of MAFbx. The present study explored the molecular determinants for this time-dependent effect using microarray analysis to identify genes that were differentially regulated by administration of nandrolone for 7 days beginning either concomitantly with denervation (7 days) or 29 days later (35 days)

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

12 Samples

Download data: CEL, TXT

(Submitter supplied) Purpose: The goal of this study was to determine the gene expression changes that occur over 7 days in parralyzed muscle in response to isometric contraction elicited by electrical stimulation initiated 4 months after spinal cord injury and to compare such changes to those observed in a normal muscle subjected to overload.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL6247

33 Samples

Download data: CEL

(Submitter supplied) The right legs of 8 Brown-Norway male rats were denervated by a high sciatic nerve section in the hip region of the hind limb.Two months after denervation (6 months of age), extensor digitorum longus (EDL) muscles were removed from the operated legs. The EDL muscles from 8 age-matched non-operated rats served as innervated controls. Total RNA was isolated, labeled cDNA was prepared and hybridized to the Rat Atlas 1.2 Array II membranes (Clontech Laboratories, Palo Alto, CA). more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL154

16 Samples

Download data

(Submitter supplied) The right legs of 3 months old CD1 mice were denervated by unilaterally cutting the sciatic nerve at the level of trochanter, resulting in a permanent denervation of the lower hindleg. At the indicated times, animals were killed by cervical dislocation and tibialis anterior (TA) muscles were dissected out and frozen in liquid nitrogen. The expression analysis was carried out at five time points, namely just after denervation (time 0) and 1, 3, 7 and 14 days after nerve interruption. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1523

10 Samples

Download data: TIFF

(Submitter supplied) 2,061 muscle-specific cDNA probes (300-600 bp), restricted to the 3'-end of each mRNA, were spotted in quadruplicates. MICROARRAY FABRICATION: The cDNA inserts were cloned in the plasmid vector pZErO-1 (Invitrogen) and amplified by PCR with universal primers (A: 5’-TCCGGCTCGTATGTTGTGTGG-3’, B: 5’-GTTGTAAAACGACGGCCAGTG-3’). The quality of each amplicon was monitored on agarose gels: cDNA probes showing more than a single size product, described by a ‘WARNING’ message, were excluded from subsequent microarray analyses. more...

Organism:

Mus musculus

1 Series

10 Samples

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(Submitter supplied) Endurance and resistance exercise training induce specific and profound changes in the skeletal muscle transcriptome. PGC-1a; coactivators are not only among the genes differentially induced by distinct training methods, but also participate in the ensuing signaling cascades that allow skeletal muscle to adapt to each type of exercise. While endurance training preferentially induces PGC-1a1 expression, resistance exercise activates the expression of PGC-1a2, a3, and a4. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6096

15 Samples

Download data: CEL

(Submitter supplied) We investigated the short and long term effects of electrically induced exercise on mRNA expression of human paralyzed muscle. We developed an exercise dose that activated the muscle for 0.6% of the day. The short term effects were assessed 3 hours after a single dose of exercise, while the long term effects were assessed after training 5 days per week for at least one year (adherence 81%). A single dose of electrical stimulation increased the mRNA expression of transcriptional, translational, and enzyme regulators of metabolism important to shift muscle toward an oxidative phenotype (PGC-1a, NR4A3, IFRD1, ABRA, PDK4). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5175

20 Samples

Download data

(Submitter supplied) Transcripts were measured in infraspinatus muscle before (PRE) and after 16 weeks of tendon release (TR) and 6 weeks of subsequent repair (END) in the operated and contralateral (END-CC) muscle. A group of animals received intramuscular injections of nandrolone starting with the surgery (N22W). In another group injection of nandrolone was started at the time of tendon repair (N6W). The CONTROL group received no nandrolone.

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19778

36 Samples

Download data: TXT

(Submitter supplied) Three male 8-week-old Wistar rats were used. The peroneal nerve of the animals was transected and sutured to the biceps femoris muscle. Four weeks later, the proximal stump was sutured to the distal stump. The animals survived for 8 weeks. All experiments were performed under Ketanest (ketamine hydrochloride, Essex Pharma GmbH, Munich, Germany; 100 mg/kg) and Rompun (xylazine hydrochloride, Bayer, Leverkusen, Germany; 10 mg/kg) anesthesia. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS2243

Platform:

GPL341

6 Samples

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Analysis of reinnervated tibialis anterior muscles that were previously denervated for 4 weeks. Denervation-induced myofiber atrophy can be reversed by reinnervation. Results provide insight into the molecular mechanisms contributing to myofiber growth after reinnervation.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL341

Series:

GSE2566

6 Samples

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(Submitter supplied) Skeletal muscle exhibits remarkably plasticity under both physiological and pathological conditions. We used adult mice with sciatic denervation as model of muscle atrophy. SnRNA-seq was performed to generate single-nucleus transcriptome profiles of gastrocnemius from normal and denervation mice. Our results define the myonuclear transition, metabolic remodeling and gene regulation networks associated with muscle atrophy induced by denervation and illustrated the molecular basis of heterogeneity and plasticity of muscle cells in response to muscle atrophy, thus providing a resource for exploring molecular mechanisms leading to muscle atrophy

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

6 Samples

Download data: RDS, TAR

(Submitter supplied) Loss of muscle proteins and the consequent weakness has important clinical consequences in diseases such as cancer, diabetes, chronic heart failure and in ageing. In fact, excessive proteolysis causes cachexia, accelerates disease progression and worsens life expectancy. Muscle atrophy involves a common pattern of transcriptional changes in a small subset of genes named atrophy-related genes or atrogenes. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10688

18 Samples

Download data: CSV

(Submitter supplied) Loss of muscle proteins and the consequent weakness has important clinical consequences in diseases such as cancer, diabetes, chronic heart failure and in ageing. In fact, excessive proteolysis causes cachexia, accelerates disease progression and worsens life expectancy. Muscle atrophy involves a common pattern of transcriptional changes in a small subset of genes named atrophy-related genes or atrogenes. more...

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL17835

39 Samples

Download data: CSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6105

48 Samples

Download data

(Submitter supplied) Analysis of dexamethasone-regulation of muscle mass at gene expression level. The hypothesis tested in the present study was that the presence of MuRF1 contributes to the extent of gene expression changes observed in specific sets of genes during a challenge leading to muscle atrophy. Results provide important information on the response of triceps surae muscle to synthetic glucocorticoid treatment, such as specific cell signaling and metabolic enzyme genes, that may be influenced by MuRF1 during atrophy.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6105

24 Samples

Download data: TXT

(Submitter supplied) Analysis of denervation induced regulation of muscle mass at gene expression level. The hypothesis tested in the present study was that the presence of MuRF1 contributes to the extent of gene expression changes observed in specific sets of genes during a challenge leading to muscle atrophy. Results provide important information on the response of triceps surae muscle to sciatic nerve resection (denervation), such as specific structural, metabolic, and neuromuscular junction associated genes, that may be influenced by MuRF1 during atrophy.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6105

24 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Methylation profiling by high throughput sequencing

Platform:

GPL24247

64 Samples

Download data: TXT

(Submitter supplied) Spinal cord injury (SCI) results in rapid muscle loss in the acute timeframe post-injury. The principal mechanisms of general muscle atrophy have been well-described but there is limited information available specific to SCI. Exogenous drug/small molecule interventions to slow muscle atrophy in severe-to-complete SCI have been relatively ineffective due to the unique combination in loss of central nervous system function due to the spinal lesion, systemic response to the trauma itself, and resultant paralysis. more...

Organism:

Mus musculus

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL24247

32 Samples

Download data: TXT

(Submitter supplied) Spinal cord injury (SCI) results in rapid muscle loss in the acute timeframe post-injury. The principal mechanisms of general muscle atrophy have been well-described but there is limited information available specific to SCI. Exogenous drug/small molecule interventions to slow muscle atrophy in severe-to-complete SCI have been relatively ineffective due to the unique combination in loss of central nervous system function due to the spinal lesion, systemic response to the trauma itself, and resultant paralysis. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

32 Samples

Download data: XLSX

(Submitter supplied) Glucocorticoids are a well recognized and common cause of muscle atrophy. Glucocorticoid-induced atrophy can be prevented by testosterone, but the molecular mechanisms underlying such protection have not been described. Thus, the global effects of testosterone on dexamethasone-induced changes in gene expression were evaluated in rat gastrocnemius muscle using Affymetrix 230_2 DNA microarrays. Gene expression was analyzed after 7 days administration of dexamethasone, dexamethasone plus testosterone, or vehicle. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

9 Samples

Download data: CEL