GEO DataSets

(Submitter supplied) Transcriptional analysis using RNA-Seq to low oxygen

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16530

6 Samples

Download data: TXT

(Submitter supplied) Purpose: The goals of this study are to find out the genes regulated directly or indirectly by C2H2 transcription factors Vrf1 by RNA-seq in the rice blast fungus Magnaporthe oryzae Methods: Appressorial mRNAs of the wild type strain 70-15 and Δvrf1 conidia incubated in H2O on plastic membranes at 28˚C for 5 h, in triplicate independently for each strain, were extracted and isolated by RNeasy Plant mini kit (QIAGEN) and AMPure XP beads (Beckman). more...

Organism:

Pyricularia oryzae 70-15

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21479

6 Samples

Download data: TXT

(Submitter supplied) Purpose: The goals of this study are to find out the genes regulated directly or indirectly by C2H2 transcription factors Vrf1 and Vrf2 by RNA-seq and to evaluate the similarities and differences in gene regulation mechanisms of Vrf1 and Vrf2 in the rice blast fungus Methods: Mycelial mRNAs of the wild type strain 70-15, Δvrf1 and Δvrf2 incubated in H2O at 25˚C for 4 h, in triplicate independently for each strain, were extracted and isolated by RNeasy Plant mini kit (QIAGEN) and AMPure XP beads (Beckman). more...

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18618

9 Samples

Download data: TXT

(Submitter supplied) Purpose: The goals of this study are to find out the genes regulated directly or indirectly by the regulatory factor X protein MoRfx1 by RNA-seq in the rice blast fungus Methods: Mycelial mRNAs of the wild type strain 70-15, ΔMorfx1 incubated in H2O at 25˚C for 4 h, in triplicate independently for each strain, were extracted and isolated by RNeasy Plant mini kit (QIAGEN) and AMPure XP beads (Beckman). more...

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18618

6 Samples

Download data: TXT

(Submitter supplied) RNAi is a conserved mechanism in eukaryotic organisms and has been the subject of recent attention pertaining to many animal and plant models. But research related to fungal systems is limited, especially in plant pathogenic models. The present study was undertaken to examine the involvement of RNAi components on the biogenesis of small RNAs and their impact on growth and pathogenicity of the plant pathogenic model organism Magnaporthe oryzae. more...

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL16530

36 Samples

Download data: TXT

(Submitter supplied) Mycelium from the rice blast fungus Magnaporthe oryzae was grown in both rich medium and under nutrient limiting conditions. Genes were identified that were more highly expressed in one condition as compared to the other.

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13743

2 Samples

Download data: TXT

(Submitter supplied) Mycelium from the rice blast fungus Magnaporthe oryzae was grown in both rich medium and under nutrient limiting conditions. Genes were identified that were more highly expressed in one condition as compared to the other.

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13743

6 Samples

Download data: TXT

(Submitter supplied) The rice-blast fungus Magnaporthe oryzae is disseminated using a three-celled spore or conidium. Upon landing on the surface of a rice leaf, the conidium germinates and forms a specialised structure for entry into the host plant - the appressorium. In this study we have followed gene expression thoughout germination of the conidium and formation of the appressorium to identify genes that may be important for appressorium function. more...

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13743

12 Samples

Download data: TXT

(Submitter supplied) The rice blast fungus, Magnaporthe oryzae is a destructive pathogen of rice and other related crops, causing significant yield losses worldwide. Endogenous small RNAs (sRNAs), including small interfering RNAs (siRNAs) and microRNAs (miRNAs) are critical components of gene regulation in many eukaryotic organisms. Recently several new species of sRNAs have been identified in fungi. This fact along with the availability of genome sequence makes M. more...

Organism:

Oryza sativa; Pyricularia oryzae

Type:

Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL9316 GPL16451

8 Samples

Download data: TXT

(Submitter supplied) In this study we identified the L-arabinose-responsive regulator of Pyricularia oryzae that regulates L-arabinose release and catabolism. Previously we identified the Zn2Cys6 transcription factor (TF) AraR that has this role in the Trichocomaceae family (Eurotiales), but is absent in other fungi. Candidate Zn2Cys6 TF genes were selected according to their transcript profiles on L-arabinose. Deletion mutants of these genes were screened for their growth phenotype on L-arabinose. more...

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16530

6 Samples

Download data: XLS

(Submitter supplied) This experiment was designed to analyze transcriptional changes that occur when the fungal plant pathogen M. grisea is shifted from a complete media to a nitrogen-starved media. Comparisons were made in the following way between fungal mycelial grown in minimal media supplemented with a nitrogen source (MM+N) and minimal media without a nitrogen source (NS) Keywords: parallel sample

Organism:

Pyricularia grisea

Type:

Expression profiling by array

Platform:

GPL889

6 Samples

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(Submitter supplied) Rice blast is the most threatening disease to cultivated rice worldwide. Magnaporthe oryzae, its causal agent is likely to encounter environmental challenges during invasive growth in the host plants that require shifts in gene expression to establish a compatible interaction. Here, we tested the hypothesis that M. oryzae have similar gene expression patterns in planta, versus during in vitro stresses. more...

Organism:

Pyricularia grisea; Pyricularia oryzae

Type:

Expression profiling by array

Platform:

GPL9203

16 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Oryza sativa; Oryza sativa Japonica Group; Pyricularia grisea; Pyricularia oryzae

Type:

Expression profiling by array

Platforms:

GPL2874 GPL892

24 Samples

Download data: TXT

(Submitter supplied) The hemibiotrophic fungus Magnaporthe oryzae produces specialized biotrophic invasive hyphae (IH) that alter membrane structure and defense responses in invaded rice cells. IH successively invade live neighbor cells, apparently through plasmodesmata. Understanding fungal and rice genes that contribute to biotrophic invasion has been a challenge because so few plant cells have encountered IH at the earliest infection stages. more...

Organism:

Oryza sativa; Oryza sativa Japonica Group; Pyricularia oryzae

Type:

Expression profiling by array

Platform:

GPL892

12 Samples

Download data: TXT

(Submitter supplied) The hemibiotrophic fungus Magnaporthe oryzae produces specialized biotrophic invasive hyphae (IH) that alter membrane structure and defense responses in invaded rice cells. IH successively invade live neighbor cells, apparently through plasmodesmata. Understanding fungal and rice genes that contribute to biotrophic invasion has been a challenge because so few plant cells have encountered IH at the earliest infection stages. more...

Organism:

Pyricularia grisea; Pyricularia oryzae; Oryza sativa

Type:

Expression profiling by array

Platform:

GPL2874

12 Samples

Download data: TXT

(Submitter supplied) Plants and animals have evolved a first line of defense response to pathogens called innate or basal immunity. While basal defenses in these organisms are well studied, there is almost a complete lack of understanding of such systems in fungal species, and more specifically, how they are able to detect and mount a defense response upon pathogen attack. Hence, the goal of the present study was to understand how fungi respond to biotic stress by assessing the transcriptional profile of the rice blast pathogen, Magnaporthe oryzae, when challenged with the bacterial antagonist Lysobacter enzymogenes. more...

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16530

12 Samples

Download data: TXT, XLSX

(Submitter supplied) Magnaporthe oryzae causes rice blast, the most devastating foliar fungal disease of cultivated rice. During disease development the fungus simultaneously maintains both biotrophic and necrotrophic growth corresponding to a hemi-biotrophic life style. The ability of M. oryzae to also colonize roots and subsequently develop blast symptoms on aerial tissue has been recognized. The fungal root infection strategy and the respective host responses are currently unknown. more...

Organism:

Oryza sativa

Type:

Expression profiling by array

Platform:

GPL2025

12 Samples

Download data: CEL

(Submitter supplied) Purpose: The goals of this study are to find out the genes regulated directly or indirectly by Zn2Cys6 transcription factors Cnf2 and Gpf1 by RNA-seq and to evaluate the similarities and differences in gene regulation mechanisms of Cnf2 and Gpf1 in the rice blast fungus Methods: Mycelial mRNAs of the wild type strain 70-15, mutants Δgpf1 (01F1-1) and Δcnf2 (03C7-2) incubated in H2O at 25˚C for 4 h, in triplicate independently for each strain, were extracted and isolated by RNeasy Plant mini kit (QIAGEN) and AMPure XP beads (Beckman). more...

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18618

9 Samples

Download data: TXT

(Submitter supplied) Purpose: The goals of this study are to find out the genes regulated directly or indirectly by bHLH protein MoBhlh6 (Crf1) by RNA-seq in the rice blast fungus Methods: Mycelial mRNAs of the wild type strain 70-15, ΔMobhlh6 incubated in H2O at 25˚C for 4 h, in triplicate independently for each strain, were extracted and isolated by RNeasy Plant mini kit (QIAGEN) and AMPure XP beads (Beckman). RNA-sequencing libraries were constructed using NEBNext RNA sample preparation kit (NEB). more...

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18618

6 Samples

Download data: TXT