GEO DataSets

(Submitter supplied) RNAi is a conserved mechanism in eukaryotic organisms and has been the subject of recent attention pertaining to many animal and plant models. But research related to fungal systems is limited, especially in plant pathogenic models. The present study was undertaken to examine the involvement of RNAi components on the biogenesis of small RNAs and their impact on growth and pathogenicity of the plant pathogenic model organism Magnaporthe oryzae. more...

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL16530

36 Samples

Download data: TXT

(Submitter supplied) The rice blast fungus, Magnaporthe oryzae is a destructive pathogen of rice and other related crops, causing significant yield losses worldwide. Endogenous small RNAs (sRNAs), including small interfering RNAs (siRNAs) and microRNAs (miRNAs) are critical components of gene regulation in many eukaryotic organisms. Recently several new species of sRNAs have been identified in fungi. This fact along with the availability of genome sequence makes M. more...

Organism:

Oryza sativa; Pyricularia oryzae

Type:

Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL9316 GPL16451

8 Samples

Download data: TXT

(Submitter supplied) Transcriptional analysis using RNA-Seq to low oxygen

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16530

6 Samples

Download data: TXT

(Submitter supplied) In this study we identified the L-arabinose-responsive regulator of Pyricularia oryzae that regulates L-arabinose release and catabolism. Previously we identified the Zn2Cys6 transcription factor (TF) AraR that has this role in the Trichocomaceae family (Eurotiales), but is absent in other fungi. Candidate Zn2Cys6 TF genes were selected according to their transcript profiles on L-arabinose. Deletion mutants of these genes were screened for their growth phenotype on L-arabinose. more...

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16530

6 Samples

Download data: XLS

(Submitter supplied) Purpose: The goals of this study are to find out the genes regulated directly or indirectly by Zn2Cys6 transcription factors Cnf2 and Gpf1 by RNA-seq and to evaluate the similarities and differences in gene regulation mechanisms of Cnf2 and Gpf1 in the rice blast fungus Methods: Mycelial mRNAs of the wild type strain 70-15, mutants Δgpf1 (01F1-1) and Δcnf2 (03C7-2) incubated in H2O at 25˚C for 4 h, in triplicate independently for each strain, were extracted and isolated by RNeasy Plant mini kit (QIAGEN) and AMPure XP beads (Beckman). more...

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18618

9 Samples

Download data: TXT

(Submitter supplied) Digital gene expression profiling analysis of the mycelium growth and conidiogenesis stages of Metarhizium robertsii

Organism:

Metarhizium robertsii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16039

4 Samples

Download data: XLSX

(Submitter supplied) Plants and animals have evolved a first line of defense response to pathogens called innate or basal immunity. While basal defenses in these organisms are well studied, there is almost a complete lack of understanding of such systems in fungal species, and more specifically, how they are able to detect and mount a defense response upon pathogen attack. Hence, the goal of the present study was to understand how fungi respond to biotic stress by assessing the transcriptional profile of the rice blast pathogen, Magnaporthe oryzae, when challenged with the bacterial antagonist Lysobacter enzymogenes. more...

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16530

12 Samples

Download data: TXT, XLSX

(Submitter supplied) Fusarium graminearum (teleomorph Gibberella zeae) is a prominent pathogen that infects major cereal crops, such as wheat, barley, and maize. To dissect molecular mechanisms of small non-coding RNA-mediated gene regulation during ascospore production, we compared small RNA transcriptomes of fungal cultures harvested from F. graminearum wild-type strain Z-3639 and RNAi component mutants at 5 days after sexual induction.

Organism:

Fusarium graminearum

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL17573

7 Samples

Download data: TXT

(Submitter supplied) Fusarium graminearum (teleomorph Gibberella zeae) is a prominent pathogen that infects major cereal crops, such as wheat, barley, and maize. To dissect molecular mechanisms of small non-coding RNA-mediated gene regulation during ascospore production, we compared transcriptomes of fungal cultures harvested from F. graminearum wild-type strain Z-3639 and RNAi component mutants at 5 days after sexual induction.

Organism:

Fusarium graminearum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17573

7 Samples

Download data: TXT

(Submitter supplied) Antisense (as)lncRNAs are extensively degraded by the nuclear exosome and the cytoplasmic exoribonuclease Xrn1 in the budding yeast Saccharomyces cerevisiae, lacking RNA interference (RNAi). Whether the ribonuclease III Dicer affects aslncRNAs in close RNAi-capable relatives remains unknown. Using genome-wide RNA profiling, here we show that aslncRNAs are primarily targeted by the exosome and Xrn1 in the RNAi-capable budding yeast Naumovozyma castellii, Dicer only affecting Xrn1-sensitive lncRNAs (XUTs) levels in Xrn1-deficient cells. more...

Organism:

Naumovozyma castellii

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL25506

1 Sample

Download data: XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Naumovozyma castellii

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL25506

17 Samples

Download data

(Submitter supplied) Antisense (as)lncRNAs are extensively degraded by the nuclear exosome and the cytoplasmic exoribonuclease Xrn1 in the budding yeast Saccharomyces cerevisiae, lacking RNA interference (RNAi). Whether the ribonuclease III Dicer affects aslncRNAs in close RNAi-capable relatives remains unknown. Using genome-wide RNA profiling, here we show that aslncRNAs are primarily targeted by the exosome and Xrn1 in the RNAi-capable budding yeast Naumovozyma castellii, Dicer only affecting Xrn1-sensitive lncRNAs (XUTs) levels in Xrn1-deficient cells. more...

Organism:

Naumovozyma castellii

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL25506

8 Samples

Download data: XLSX

(Submitter supplied) Antisense (as)lncRNAs are extensively degraded by the nuclear exosome and the cytoplasmic exoribonuclease Xrn1 in the budding yeast Saccharomyces cerevisiae, lacking RNA interference (RNAi). Whether the ribonuclease III Dicer affects aslncRNAs in close RNAi-capable relatives remains unknown. Using genome-wide RNA profiling, here we show that aslncRNAs are primarily targeted by the exosome and Xrn1 in the RNAi-capable budding yeast Naumovozyma castellii, Dicer only affecting Xrn1-sensitive lncRNAs (XUTs) levels in Xrn1-deficient cells. more...

Organism:

Naumovozyma castellii

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL25506

8 Samples

Download data: XLSX