GEO DataSets

(Submitter supplied) A combined transcriptomic and miRNA-based analysis of the molecular mechanism of collection preference in Italian honey bees was conducted, mainly selecting the long-range sensor tentacles and the proximal sensor mouthparts.

Organism:

Apis mellifera

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27915

18 Samples

Download data: TXT

(Submitter supplied) RNA sequencing of Apis mellifera abdominal fat body and matched whole brain following a knockdown in fat body ame-miR-305-5p expression

Organism:

Apis mellifera

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27915

138 Samples

Download data: TXT

(Submitter supplied) Down syndrome cell adhesion molecule (Dscam) gene encodes a cell adhesion molecule required for neuronal wiring. A remarkable feature of invertebrate Dscam is massive alternative splicing generating thousands of different isoforms from three variable clusters of alternative exons. Dscam expression and diversity arising from alternative splicing have been studied during development, but whether they exert functions in differentiated brains has not been determined. more...

Organism:

Apis mellifera

Type:

Other

Platform:

GPL27915

1 Sample

Download data: XLSX

(Submitter supplied) We provide a detailed analysis of miRNA in the royal jelly nanovesicles.

Organism:

Apis mellifera

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL32906

3 Samples

Download data: CSV

(Submitter supplied) RNA-Sequencing performed on 177 honey bee whole-brains, divided into "soldier" and "forager" groups from Puerto Rican honey bee colonies.

Organism:

Apis mellifera

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27915

177 Samples

Download data: TXT

(Submitter supplied) Each sample includes 5 pooled whole-brains from adult worker honey bees collected across two behavioral groups: soldiers and foragers. We used the Chromium Single Cell 3' Reagent Kits with the Chromium Controller (10x Genomics) and performed sequencing on the Illumina NovaSeq 6000.

Organism:

Apis mellifera

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27915

8 Samples

Download data: TAR

(Submitter supplied) The effects of neonicotinoid insecticides (NNIs) on honey bee health is intensely debated, with numerous studies showing negative effects of exposure, while others report no such effects. Understanding the cause of these differences is critical for developing evidence-based policy on the use of NNIs. We carried out experiments to study the genetic and molecular basis of NNI tolerance in honey bees, which may underlie the discrepancies observed in the literature. more...

Organism:

Apis mellifera

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27915

18 Samples

Download data: TXT

(Submitter supplied) Overwintering mortality is the greatest threat to the supply of healthy bee colonies available for pollination of spring-blooming crops, but efforts to mitigate colony losses are hindered because underlying mechanisms remain poorly understood. In the present study, we identified that sirtuin signaling pathway is the most significantly affected pathway in collapsing overwintering colonies that carried a high prevalence of pathogens and is a convergent signaling hub that links mitochondrial dysfunction and metabolism alterations. more...

Organism:

Apis mellifera

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19174

6 Samples

Download data: GTF, TXT

(Submitter supplied) Examination of Mblk-1-binding DNA regions in honey bee adult mushroom bodies(MBs) and pupal whole brains

Organism:

Apis mellifera

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19174

8 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Apis mellifera

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL27915

39 Samples

Download data: NARROWPEAK, TSV

(Submitter supplied) Using the assay for transposase-accessible chromatin by sequencing (ATAC-seq), we produce the first genome- wide maps of chromatin accessibility across all three adult honey bee phenotypes. We identify ~ 3000 regulatory regions in queen, ~ 4500 in worker and ~ 4000 in drone, with the vast majority of these sites located within intronic regions. Integrating ATAC-seq, RNA-seq and ChIP-seq data, we show a positive correlation between chromatin accessibility at introns, flanking H3K27ac modified nucleosomes and abundance of the respective mRNA transcript. more...

Organism:

Apis mellifera

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27915

6 Samples

Download data: TSV, TXT

(Submitter supplied) Using the assay for transposase-accessible chromatin by sequencing (ATAC-seq), we produce the first genome- wide maps of chromatin accessibility across all three adult honey bee phenotypes. We identify ~ 3000 regulatory regions in queen, ~ 4500 in worker and ~ 4000 in drone, with the vast majority of these sites located within intronic regions. Integrating ATAC-seq, RNA-seq and ChIP-seq data, we show a positive correlation between chromatin accessibility at introns, flanking H3K27ac modified nucleosomes and abundance of the respective mRNA transcript. more...

Organism:

Apis mellifera

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL27915

27 Samples

Download data: NARROWPEAK

(Submitter supplied) Using the assay for transposase-accessible chromatin by sequencing (ATAC-seq), we produce the first genome- wide maps of chromatin accessibility across all three adult honey bee phenotypes. We identify ~ 3000 regulatory regions in queen, ~ 4500 in worker and ~ 4000 in drone, with the vast majority of these sites located within intronic regions. Integrating ATAC-seq, RNA-seq and ChIP-seq data, we show a positive correlation between chromatin accessibility at introns, flanking H3K27ac modified nucleosomes and abundance of the respective mRNA transcript. more...

Organism:

Apis mellifera

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL27915

6 Samples

Download data: NARROWPEAK

(Submitter supplied) The honeybee (Apis mellifera) is a well-known eusocial insect. In honeybee colonies, thousands of sterile workers, including nurse and forager bees, perform various tasks within or outside the hive, respectively. The queen is the only fertile female and is responsible for reproduction. The queen and workers share similar genomes, but occupy different caste statuses. We established single-cell transcriptomic atlases of brains from queens and worker subcastes, and identified five major cell groups: Kenyon, optic lobe, olfactory projection, glial, and hemocyte cells. more...

Organism:

Apis mellifera

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27915

12 Samples

Download data: MTX, TSV, TXT

(Submitter supplied) Here we present the first characterisation of small RNAs in honey bee reproductive tissues. We conclude that small RNAs are likely to play an integral role in honey bee gametogenesis and reproduction and provide a plausible mechanism for parent-of origin-effects on gene expression and reproductive physiology. present in honey bee reproductive tissues: ovaries, spermatheca, semen, fertilised and unfertilised eggs, and testes.

Organism:

Apis mellifera

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL16097

24 Samples

Download data: TXT

(Submitter supplied) A.mellifera ligustica with similar group potential and randomly divided them into two sets (3 replicates in each group). One set as control groups, and the other set as test groups. The test groups were exposed to fluvalinate at a normal dosage for administration to honeybees (according to the instructions for use of the medicine, mite treatment was added every 7 days). The sequencing is performed by the Illumina HiSeq TM 4000.

Organism:

Apis mellifera ligustica

Type:

Expression profiling by high throughput sequencing

Platform:

GPL31289

11 Samples

Download data: XLSX

(Submitter supplied) RNA-sequencing of gut and different brain regions of gnotobiotic honeybees

Organism:

Apis mellifera

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24017

154 Samples

Download data: TXT

(Submitter supplied) Neonicotinoid insecticides have been implicated in honey bee declines, with many studies showing that sub-lethal exposure impacts bee behaviors such as foraging, learning and memory. Despite the large number of ecotoxicological studies carried out to date, most focus on a handful of worker phenotypes leading to a ‘streetlight effect’ where the a priori choice of phenotypes to measure may influence the results and conclusions arising from the studies. This bias can be overcome with the use of toxicological transcriptomics, where changes in gene expression can provide a more objective view of how pesticides alter animal physiology. more...

Organism:

Apis mellifera

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24017

79 Samples

Download data: TXT

(Submitter supplied) Seeking to further characterize the effect of Halofuginone in honey bees and to better understand cellular stress pathways in this species more generally, we used RNAseq to identify novel changes in gene expression after its ingestion and observed a striking induction of genes involved in ribosome biogenesis, translation, tRNA synthesis, and ribosome-associated quality control (RQC). Feeding bees cycloheximide (CHX) to halt ribosome translocation also induced all tested target genes, suggesting that this gene expression program could be induced by ribosome stalling in addition to tRNA-synthetase inhibition. more...

Organism:

Apis mellifera

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16097

6 Samples

Download data: CSV

(Submitter supplied) Various bioactive food compounds may confer health and longevity benefits, possibly through altering or preserving the human epigenome. While bioactive food compounds are widely being marketed as ‘improving health and longevity’ by counteracting harmful effects of poor nutrition and lifestyle, claimed effects are often not adequately documented. Using the honey bee (Apis mellifera) as a model species, we here employed a multi-step screening approach to investigate seven compounds for effects on lifespan and DNA methylation using ELISA and whole genome bisulfite sequencing (WGBS). more...

Organism:

Apis mellifera

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL19174

13 Samples

Download data: CSV