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| Status |
Public on May 09, 2023 |
| Title |
Single-cell dissection of aggression in honeybee colonies |
| Organism |
Apis mellifera |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Each sample includes 5 pooled whole-brains from adult worker honey bees collected across two behavioral groups: soldiers and foragers. We used the Chromium Single Cell 3' Reagent Kits with the Chromium Controller (10x Genomics) and performed sequencing on the Illumina NovaSeq 6000.
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| Overall design |
We dissected and pooled 5 whole-brains from soldiers and forager adult worker honey bees. We collected one soldier and one forager sample (5 brains per sample) from four colonies (eight samples total, two per colony) headed by naturally mated queens at the University of Illinois at Urbana-Champaign Bee Research Facility in Urbana, IL. All samples were collected in August 2019.
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| |
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| Contributor(s) |
Traniello IM |
| Citation(s) |
37264201 |
| |
| Submission date |
Dec 14, 2019 |
| Last update date |
Aug 08, 2023 |
| Contact name |
Ian Michael Traniello |
| E-mail(s) |
it4770@princeton.edu
|
| Organization name |
Princeton University
|
| Department |
Lewis-Sigler Insitute for Genomic Biology
|
| Lab |
Sarah Kocher
|
| Street address |
Princeton University
|
| City |
Princeton |
| State/province |
NJ |
| ZIP/Postal code |
08544 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL27915 |
Illumina NovaSeq 6000 (Apis mellifera) |
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| Samples (8)
|
| GSM4218160 |
5 pooled forager brains, samples collected from Colony O2 |
| GSM4218161 |
5 pooled forager brains, samples collected from Colony O22 |
| GSM4218162 |
5 pooled forager brains, samples collected from Colony O38 |
| GSM4218163 |
5 pooled forager brains, samples collected from Colony O43 |
| GSM4218164 |
5 pooled soldier brains, samples collected from Colony O2 |
| GSM4218165 |
5 pooled soldier brains, samples collected from Colony O22 |
| GSM4218166 |
5 pooled soldier brains, samples collected from Colony O38 |
| GSM4218167 |
5 pooled soldier brains, samples collected from Colony O43 |
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| Relations |
| BioProject |
PRJNA595689 |
| SRA |
SRP237631 |
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