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Spsb2 splA/ryanodine receptor domain and SOCS box containing 2 [ Mus musculus (house mouse) ]

Gene ID: 14794, updated on 8-May-2024

Summary

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Official Symbol
Spsb2provided by MGI
Official Full Name
splA/ryanodine receptor domain and SOCS box containing 2provided by MGI
Primary source
MGI:MGI:1315199
See related
Ensembl:ENSMUSG00000038451 AllianceGenome:MGI:1315199
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
C9; SSB2; Grcc9
Summary
This gene encodes a member of the SSB family of proteins that contain a central SPRY (repeats in splA and ryanodine receptors) domain and a C-terminal SOCS (suppressor of cytokine signaling) box. The encoded protein is an adaptor protein in the E3 ubiquitin ligase complex that ubiquitinates inducible nitric oxide synthase and targets it for proteasomal degradation. Mice lacking the encoded protein exhibit lower blood urea nitrogen levels and mild thrombocytopenia due to reduced platelet production. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]
Expression
Ubiquitous expression in testis adult (RPKM 25.4), adrenal adult (RPKM 14.6) and 25 other tissues See more
Orthologs
human all
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Genomic context

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Location:
6 F2; 6 59.17 cM
Exon count:
4
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 6 NC_000072.7 (124785640..124787582)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 6 NC_000072.6 (124808604..124810619)

Chromosome 6 - NC_000072.7Genomic Context describing neighboring genes Neighboring gene leucine rich repeat containing 23 Neighboring gene predicted gene, 38895 Neighboring gene STARR-seq mESC enhancer starr_17308 Neighboring gene predicted gene, 38894 Neighboring gene STARR-positive B cell enhancer ABC_E2803 Neighboring gene STARR-positive B cell enhancer mm9_chr6:124764120-124764420 Neighboring gene triosephosphate isomerase 1 Neighboring gene ubiquitin specific peptidase 5 (isopeptidase T) Neighboring gene CapStarr-seq enhancer MGSCv37_chr6:124778211-124778394 Neighboring gene cell division cycle associated 3

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. A potent cyclic peptide targeting SPSB2 protein as a potential anti-infective agent. Yap BK, et al. J Med Chem, 2014 Aug 28. PMID 25068993
  2. SPRY domain-containing SOCS box protein 2: crystal structure and residues critical for protein binding. Kuang Z, et al. J Mol Biol, 2009 Feb 27. PMID 19154741
  3. Dynamics of the SPRY domain-containing SOCS box protein 2: flexibility of key functional loops. Yao S, et al. Protein Sci, 2006 Dec. PMID 17088318, Free PMC Article
  4. Genetic deletion of murine SPRY domain-containing SOCS box protein 2 (SSB-2) results in very mild thrombocytopenia. Masters SL, et al. Mol Cell Biol, 2005 Jul. PMID 15964819, Free PMC Article
  5. TLR regulation of SPSB1 controls inducible nitric oxide synthase induction. Lewis RS, et al. J Immunol, 2011 Oct 1. PMID 21876038

See all (28) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Structural basis for the regulation of inducible nitric oxide synthase by the SPRY domain-containing SOCS box protein SPSB2, an E3 ubiquitin ligase.
    Title: Structural basis for the regulation of inducible nitric oxide synthase by the SPRY domain-containing SOCS box protein SPSB2, an E3 ubiquitin ligase.
  2. these results establish SSB1/2 as safeguards of B cell development and unveil their differential requirement in immature and mature B lymphocytes
    Title: SSB1/SSB2 Proteins Safeguard B Cell Development by Protecting the Genomes of B Cell Precursors.
  3. Collectively, Ssb1 and Ssb2 control crucial aspects of Hematopoietic stem and progenitor cells function, including proliferation and survival in vivo by resolving replicative stress to maintain genomic stability.
    Title: Ssb1 and Ssb2 cooperate to regulate mouse hematopoietic stem and progenitor cells by resolving replicative stress.
  4. structural changes limit high-affinity interactions for SPSB2 to aspartate-containing sequences
    Title: Structural basis for Par-4 recognition by the SPRY domain- and SOCS box-containing proteins SPSB1, SPSB2, and SPSB4.
  5. The SPRY domain-containing SOCS box protein SPSB2 targets iNOS for proteasomal degradation.
    Title: The SPRY domain-containing SOCS box protein SPSB2 targets iNOS for proteasomal degradation.
  6. Determined the first SPRY-domain structure of SSB-2 that adopts a novel fold and contains conserved structural determinants for binding to prostate apoptosis response protein-4 (PAR-4).
    Title: The SPRY domain of SSB-2 adopts a novel fold that presents conserved Par-4-binding residues.
  7. The binding site on SSB-2 for the prostate apoptosis response-4 (Par-4) protein has been mapped in finer detail using mutational analyses.
    Title: SPRY domain-containing SOCS box protein 2: crystal structure and residues critical for protein binding.
  8. Genetic deletion of SOCS Box Protein 2 results in a very mild thrombocytopenia.
    Title: Genetic deletion of murine SPRY domain-containing SOCS box protein 2 (SSB-2) results in very mild thrombocytopenia.
  9. Backbone dynamics of SSB-2 by means of Modelfree analysis.
    Title: Dynamics of the SPRY domain-containing SOCS box protein 2: flexibility of key functional loops.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (1) 
  • Targeted (1)  1 citation

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables ubiquitin-like ligase-substrate adaptor activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in intracellular signal transduction IEA
Inferred from Electronic Annotation
more info
  
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein ubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein ubiquitination ISO
Inferred from Sequence Orthology
more info
  
involved_in ubiquitin-dependent protein catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in ubiquitin-dependent protein catabolic process ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
part_of SCF ubiquitin ligase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
SPRY domain-containing SOCS box protein 2
Names
gene-rich cluster protein C9

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001306050.2 → NP_001292979.1  SPRY domain-containing SOCS box protein 2 isoform 1

    See identical proteins and their annotated locations for NP_001292979.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1-4 and 6-7 all encode the same isoform (1).
    Source sequence(s)
    AC142254
    Consensus CDS
    CCDS20529.1
    UniProtKB/Swiss-Prot
    O88838, Q8CBA5, Q91Z15
    UniProtKB/TrEMBL
    D3YX10
    Conserved Domains (2) summary
    cd03719
    Location:223 → 264
    SOCS_SSB2; SOCS (suppressors of cytokine signaling) box of SSB2 (SPRY domain-containing SOCS box proteins)-like proteins. SSB proteins contain a central SPRY domain and a C-terminal SOCS. SSB2 has been shown to bind to MET, the receptor protein-tyrosine kinase for ...
    cd12906
    Location:46 → 217
    SPRY_SOCS1-2-4; SPRY domain in the suppressor of cytokine signaling 1, 2, 4 families (SOCS1, SOCS2, SOCS4)

  2. NM_001306051.2 → NP_001292980.1  SPRY domain-containing SOCS box protein 2 isoform 1

    See identical proteins and their annotated locations for NP_001292980.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1-4 and 6-7 all encode the same isoform (1).
    Source sequence(s)
    AC142254
    Consensus CDS
    CCDS20529.1
    UniProtKB/Swiss-Prot
    O88838, Q8CBA5, Q91Z15
    UniProtKB/TrEMBL
    D3YX10
    Related
    ENSMUSP00000041585.4, ENSMUST00000047760.10
    Conserved Domains (2) summary
    cd03719
    Location:223 → 264
    SOCS_SSB2; SOCS (suppressors of cytokine signaling) box of SSB2 (SPRY domain-containing SOCS box proteins)-like proteins. SSB proteins contain a central SPRY domain and a C-terminal SOCS. SSB2 has been shown to bind to MET, the receptor protein-tyrosine kinase for ...
    cd12906
    Location:46 → 217
    SPRY_SOCS1-2-4; SPRY domain in the suppressor of cytokine signaling 1, 2, 4 families (SOCS1, SOCS2, SOCS4)

  3. NM_001306052.2 → NP_001292981.1  SPRY domain-containing SOCS box protein 2 isoform 1

    See identical proteins and their annotated locations for NP_001292981.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1-4 and 6-7 all encode the same isoform (1).
    Source sequence(s)
    AC142254
    Consensus CDS
    CCDS20529.1
    UniProtKB/Swiss-Prot
    O88838, Q8CBA5, Q91Z15
    UniProtKB/TrEMBL
    D3YX10
    Related
    ENSMUSP00000118347.2, ENSMUST00000143040.8
    Conserved Domains (2) summary
    cd03719
    Location:223 → 264
    SOCS_SSB2; SOCS (suppressors of cytokine signaling) box of SSB2 (SPRY domain-containing SOCS box proteins)-like proteins. SSB proteins contain a central SPRY domain and a C-terminal SOCS. SSB2 has been shown to bind to MET, the receptor protein-tyrosine kinase for ...
    cd12906
    Location:46 → 217
    SPRY_SOCS1-2-4; SPRY domain in the suppressor of cytokine signaling 1, 2, 4 families (SOCS1, SOCS2, SOCS4)

  4. NM_001306053.2 → NP_001292982.1  SPRY domain-containing SOCS box protein 2 isoform 2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (5) uses an alternate splice site in the coding region, which results in a frameshift and an early stop codon, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1. Variants 5 and 8-10 all encode the same isoform (2).
    Source sequence(s)
    AC142254
    UniProtKB/TrEMBL
    D3Z7N7
    Conserved Domains (1) summary
    cd12906
    Location:46 → 201
    SPRY_SOCS1-2-4; SPRY domain in the suppressor of cytokine signaling 1, 2, 4 families (SOCS1, SOCS2, SOCS4)

  5. NM_001409886.1 → NP_001396815.1  SPRY domain-containing SOCS box protein 2 isoform 1

    Status: VALIDATED

    Source sequence(s)
    AC142254
    UniProtKB/Swiss-Prot
    O88838, Q8CBA5, Q91Z15

  6. NM_001409887.1 → NP_001396816.1  SPRY domain-containing SOCS box protein 2 isoform 1

    Status: VALIDATED

    Source sequence(s)
    AC142254
    UniProtKB/Swiss-Prot
    O88838, Q8CBA5, Q91Z15

  7. NM_001409888.1 → NP_001396817.1  SPRY domain-containing SOCS box protein 2 isoform 2

    Status: VALIDATED

    Source sequence(s)
    AC142254

  8. NM_001409889.1 → NP_001396818.1  SPRY domain-containing SOCS box protein 2 isoform 2

    Status: VALIDATED

    Source sequence(s)
    AC142254

  9. NM_001409890.1 → NP_001396819.1  SPRY domain-containing SOCS box protein 2 isoform 2

    Status: VALIDATED

    Source sequence(s)
    AC142254

  10. NM_013539.3 → NP_038567.1  SPRY domain-containing SOCS box protein 2 isoform 1

    See identical proteins and their annotated locations for NP_038567.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1-4 and 6-7 all encode the same isoform (1).
    Source sequence(s)
    AC142254
    Consensus CDS
    CCDS20529.1
    UniProtKB/Swiss-Prot
    O88838, Q8CBA5, Q91Z15
    UniProtKB/TrEMBL
    D3YX10
    Related
    ENSMUSP00000060124.5, ENSMUST00000052727.5
    Conserved Domains (2) summary
    cd03719
    Location:223 → 264
    SOCS_SSB2; SOCS (suppressors of cytokine signaling) box of SSB2 (SPRY domain-containing SOCS box proteins)-like proteins. SSB proteins contain a central SPRY domain and a C-terminal SOCS. SSB2 has been shown to bind to MET, the receptor protein-tyrosine kinase for ...
    cd12906
    Location:46 → 217
    SPRY_SOCS1-2-4; SPRY domain in the suppressor of cytokine signaling 1, 2, 4 families (SOCS1, SOCS2, SOCS4)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000072.7 Reference GRCm39 C57BL/6J

    Range
    124785640..124787582
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036165855.1 → XP_036021748.1  SPRY domain-containing SOCS box protein 2 isoform X1

    UniProtKB/TrEMBL
    D3Z7N7
    Conserved Domains (1) summary
    cd12906
    Location:46 → 201
    SPRY_SOCS1-2-4; SPRY domain in the suppressor of cytokine signaling 1, 2, 4 families (SOCS1, SOCS2, SOCS4)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O88838.1 GenPept UniProtKB/Swiss-Prot:O88838

Gene LinkOut

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