he1.2 hatching enzyme 1, tandem dupliate 2 [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: he1.2provided by ZNC
Official Full Name: hatching enzyme 1, tandem dupliate 2provided by ZNC
Primary source: ZFIN:ZDB-GENE-030131-2100
See related: Ensembl:ENSDARG00000019122 AllianceGenome:ZFIN:ZDB-GENE-030131-2100
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: h1; he1; hce1; he1b; zHCE-1; fb96f07; wu:fb96f07; si:ch211-246m6.8
Summary: Enables metalloendopeptidase activity and zinc ion binding activity. Involved in proteolysis. Acts upstream of or within hatching. Is expressed in hatching gland cell. Orthologous to human ASTL (astacin like metalloendopeptidase). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
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Genomic context

Location:: chromosome: 22

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
106	current	GRCz11 (GCF_000002035.6)	22	NC_007133.7 (14098028..14099399)
105	previous assembly	GRCz10 (GCF_000002035.5)	22	NC_007133.6 (14073251..14074622)

Chromosome 22 - NC_007133.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The hatching of zebrafish embryo is performed by a single hatching enzyme, HE1. The substrate specificity of recombinant HE1 was determined using the egg envelope subunit proteins ZP2 and ZP3, and synthetic peptides.
Title: Purification and characterization of zebrafish hatching enzyme - an evolutionary aspect of the mechanism of egg envelope digestion.

Submit: New GeneRIF Correction

General gene information

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Markers

lpl (e-PCR), detects polymorphism
- UniSTS:205574

AI397063 (e-PCR)
- UniSTS:206678

fb96f07 (e-PCR)
- UniSTS:189117

fb51b06.x1 (e-PCR)
- UniSTS:203263

Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables metalloendopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metalloendopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables metalloendopeptidase activity	IEA Inferred from Electronic Annotation more info
enables metallopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables metallopeptidase activity	IEA Inferred from Electronic Annotation more info
enables peptidase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within hatching	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within proteolysis	IDA Inferred from Direct Assay more info	PubMed
involved_in proteolysis	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within proteolysis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in extracellular region	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: hatching enzyme 1.2

Names: HCE homologue 1; choriolysin H homolog 1; hatching enzyme 1b; high choriolytic enzyme; high choriolytic enzyme 1 homolog

NP_998800.2

EC 3.4.24.67

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_213635.2 → NP_998800.2 hatching enzyme 1.2 precursor

See identical proteins and their annotated locations for NP_998800.2

Status: VALIDATED

Source sequence(s)

BC133961, EB855773

UniProtKB/Swiss-Prot

Q1LW01, Q75NR9

UniProtKB/TrEMBL

Q4G0A2

Related

ENSDARP00000009264.7, ENSDART00000018432.9

Conserved Domains (1) summary

cd04283
Location:81 → 263

ZnMc_hatching_enzyme; Zinc-dependent metalloprotease, hatching enzyme-like subfamily. Hatching enzymes are secreted by teleost embryos to digest the egg envelope or chorion. In some teleosts, the hatching enzyme may be a system consisting of two evolutionary related ...