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vsp voltage-sensor containing phosphatase [ Ciona intestinalis (vase tunicate) ]

Gene ID: 619223, updated on 21-Dec-2023

Summary

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Gene symbol
vsp
Gene description
voltage-sensor containing phosphatase
See related
Ensembl:ENSCING00000011484
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Ciona intestinalis
Lineage
Eukaryota; Metazoa; Chordata; Tunicata; Ascidiacea; Phlebobranchia; Cionidae; Ciona
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Genomic context

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See vsp in Genome Data Viewer
Location:
chromosome: 2
Exon count:
11
Annotation release Status Assembly Chr Location
104 current KH (GCF_000224145.3) 2 NC_020167.2 (3291294..3296342, complement)

Chromosome 2 - NC_020167.2Genomic Context describing neighboring genes Neighboring gene Golgi-associated plant pathogenesis-related protein 1-like Neighboring gene Golgi-associated plant pathogenesis-related protein 1-like Neighboring gene RING finger protein 150 Neighboring gene poly(U)-specific endoribonuclease-like

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_020167.2 Chromosome 2 Reference KH Primary Assembly

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Voltage-dependent motion of the catalytic region of voltage-sensing phosphatase monitored by a fluorescent amino acid. Sakata S, et al. Proc Natl Acad Sci U S A, 2016 Jul 5. PMID 27330112, Free PMC Article
  2. Sensing charges of the Ciona intestinalis voltage-sensing phosphatase. Villalba-Galea CA, et al. J Gen Physiol, 2013 Nov. PMID 24127524, Free PMC Article
  3. Tuning the voltage-sensor motion with a single residue. Lacroix JJ, et al. Biophys J, 2012 Aug 8. PMID 22947880, Free PMC Article
  4. Coupling of Ci-VSP modules requires a combination of structure and electrostatics within the linker. Hobiger K, et al. Biophys J, 2012 Mar 21. PMID 22455914, Free PMC Article
  5. Coupling of the phosphatase activity of Ci-VSP to its voltage sensor activity over the entire range of voltage sensitivity. Sakata S, et al. J Physiol, 2011 Jun 1. PMID 21486809, Free PMC Article

See all (14) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The voltage sensor regulates Ci-VSP catalytic activity by causing conformational changes in the entire catalytic region, without changing their distance from the plasma membrane.
    Title: Voltage-dependent motion of the catalytic region of voltage-sensing phosphatase monitored by a fluorescent amino acid.
  2. Data suggest that anionic lipids (such as phosphatidylinositol phosphates) play crucial role in localization of peripheral membrane proteins (such as Ci-VSP, auxilin-1, talin-1, and PTEN [phosphatase/tensin homolog]). [review-like article]
    Title: Interactions of peripheral proteins with model membranes as viewed by molecular dynamics simulations.
  3. We show that four arginine-to-histidine mutants, namely R223H to R232H, mediate voltage-dependent proton translocation across the membrane; these residues transit through the hydrophobic core of Ci-VSP as a function of the membrane potential.
    Title: Sensing charges of the Ciona intestinalis voltage-sensing phosphatase.
  4. Ci-VSP consists of an N-terminal transmembrane voltage sensor domain and a C-terminal phosphatase and tensin homologue (PTEN) domain, which in turn contains a phospphatase domain (PD) and a C2 domain.
    Title: Interactions of phosphatase and tensin homologue (PTEN) proteins with phosphatidylinositol phosphates: insights from molecular dynamics simulations of PTEN and voltage sensitive phosphatase.
  5. a unique voltage sensing mechanism in Ci-VSP
    Title: Tuning the voltage-sensor motion with a single residue.
  6. This provides the first insight into the direct intervention of the linker in the voltage-sensor domain-phosphatase domain coupling process.
    Title: Coupling of Ci-VSP modules requires a combination of structure and electrostatics within the linker.
  7. The voltage dependence of enzyme activity was superimposable on the Q-V curve, which indicates that the enzyme activity is tightly coupled to voltage sensor domain (VSD) movement over the entire range of membrane potentials that elicit VSD movement.
    Title: Coupling of the phosphatase activity of Ci-VSP to its voltage sensor activity over the entire range of voltage sensitivity.
  8. Ci-VSP plays a key role in voltage-induced signal transduction in cells of the digestive system and blood
    Title: Gene expression profile of Ci-VSP in juveniles and adult blood cells of ascidian.
  9. Study fused the voltage-sensing domain of Ci-VSP to a pair of fluorescent reporter proteins to generate a genetically encodable voltage-sensing fluorescent probe, the data presented gives insights into the voltage-sensing mechanism of Ci-VSP.
    Title: Biophysical characterization of the fluorescent protein voltage probe VSFP2.3 based on the voltage-sensing domain of Ci-VSP.
  10. The leftward shift of the Q-V voltage curve under prolonged depolarization was studied in the Ci-VSP by using electrophysiological and site-directed fluorescence measurements.
    Title: S4-based voltage sensors have three major conformations.
Submit: New GeneRIF Correction See all GeneRIFs (14)

General gene information

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Homology

Gene Ontology Provided by RefSeq

Function Evidence Code Pubs
enables monoatomic ion channel activity IEA
Inferred from Electronic Annotation
more info
 PubMed 
enables phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity IEA
Inferred from Electronic Annotation
more info
 PubMed 
Process Evidence Code Pubs
involved_in dephosphorylation IEA
Inferred from Electronic Annotation
more info
 PubMed 
involved_in monoatomic ion transport IEA
Inferred from Electronic Annotation
more info
 PubMed 
involved_in transmembrane transport IEA
Inferred from Electronic Annotation
more info
 PubMed 
Component Evidence Code Pubs
located_in cytosol IEA
Inferred from Electronic Annotation
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
 PubMed 

General protein information

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Preferred Names
voltage-sensor containing phosphatase

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001033826.1 → NP_001028998.1  voltage-sensor containing phosphatase

    See identical proteins and their annotated locations for NP_001028998.1

    Status: PROVISIONAL

    Source sequence(s)
    AB183035
    UniProtKB/TrEMBL
    A0A1W2VNG2, F6XHE4, Q4W8A1
    Related
    ENSCINP00000021926, ENSCINT00000022172
    Conserved Domains (4) summary
    smart00404
    Location:331 → 381
    PTPc_motif; Protein tyrosine phosphatase, catalytic domain motif
    COG2453
    Location:317 → 430
    CDC14; Protein-tyrosine phosphatase [Signal transduction mechanisms]
    pfam00520
    Location:137 → 241
    Ion_trans; Ion transport protein
    pfam10409
    Location:434 → 572
    PTEN_C2; C2 domain of PTEN tumour-suppressor protein

RefSeqs of Annotated Genomes: Ciona intestinalis Annotation Release 104 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference KH Primary Assembly

Genomic

  1. NC_020167.2 Reference KH Primary Assembly

    Range
    3291294..3296342 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version

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Research Materials