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prfA peptide chain release factor 1 [ Listeria monocytogenes EGD-e ]

Gene ID: 986736, updated on 4-Apr-2024

Summary

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Gene symbol
prfA
Gene description
peptide chain release factor 1
Locus tag
lmo2543
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Listeria monocytogenes EGD-e (strain: EGD-e)
Lineage
Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria
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Genomic context

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Sequence:
NC_003210.1 (2619415..2620491, complement)

NC_003210.1Genomic Context describing neighboring genes Neighboring gene serine hydroxymethyltransferase Neighboring gene phosphatase Neighboring gene tRNA threonylcarbamoyladenosine biosynthesis protein Neighboring gene protoporphyrinogen oxidase Neighboring gene thymidine kinase Neighboring gene homoserine kinase Neighboring gene threonine synthase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_003210.1

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A Tale of Two Transitions: The Unfolding Mechanism of the prfA RNA Thermosensor. Zhang H, et al. Biochemistry, 2020 Dec 8. PMID 33231432
  2. Free Fatty Acids Interfere with the DNA Binding Activity of the Virulence Regulator PrfA of Listeria monocytogenes. Dos Santos PT, et al. J Bacteriol, 2020 Jul 9. PMID 32393522, Free PMC Article
  3. A Novel Growth-Based Selection Strategy Identifies New Constitutively Active Variants of the Major Virulence Regulator PrfA in Listeria monocytogenes. Hansen S, et al. J Bacteriol, 2020 May 11. PMID 32179627, Free PMC Article
  4. Structural insights into glutathione-mediated activation of the master regulator PrfA in Listeria monocytogenes. Wang Y, et al. Protein Cell, 2017 Apr. PMID 28271443, Free PMC Article
  5. Structural basis for glutathione-mediated activation of the virulence regulatory protein PrfA in Listeria. Hall M, et al. Proc Natl Acad Sci U S A, 2016 Dec 20. PMID 27930316, Free PMC Article

See all (23) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. A Tale of Two Transitions: The Unfolding Mechanism of the prfA RNA Thermosensor.
    Title: A Tale of Two Transitions: The Unfolding Mechanism of the prfA RNA Thermosensor.
  2. A Novel Growth-Based Selection Strategy Identifies New Constitutively Active Variants of the Major Virulence Regulator PrfA in Listeria monocytogenes.
    Title: A Novel Growth-Based Selection Strategy Identifies New Constitutively Active Variants of the Major Virulence Regulator PrfA in Listeria monocytogenes.
  3. Free Fatty Acids Interfere with the DNA Binding Activity of the Virulence Regulator PrfA of Listeria monocytogenes.
    Title: Free Fatty Acids Interfere with the DNA Binding Activity of the Virulence Regulator PrfA of Listeria monocytogenes.
  4. By binding to PrfA in the cytosol of the host cell, GSH induces the correct fold of the HTH motifs, thus priming the PrfA protein for DNA interaction.
    Title: Structural basis for glutathione-mediated activation of the virulence regulatory protein PrfA in Listeria.
  5. Structural insights into glutathione-mediated activation of the master regulator PrfA in Listeria monocytogenes.
    Title: Structural insights into glutathione-mediated activation of the master regulator PrfA in Listeria monocytogenes.
  6. Using a mutant PrfA regulator locked ON (PrfA*) and thus causing PrfA- controlled genes to be constitutively activated, the constitutively virulent state strongly impaired L. monocytogenes performance in soil, the natural habitat of these bacteria.
    Title: PrfA regulation offsets the cost of Listeria virulence outside the host.
  7. Data show that a mutation that renders the virulence gene activator PrfA constitutively active not only affects the phosphorylation level of numerous proteins but also allowed the detection of about 100 novel phosphoproteins.
    Title: Quantitative proteome analyses identify PrfA-responsive proteins and phosphoproteins in Listeria monocytogenes.
  8. Constitutive activation of PrfA potentiated virulence of the pathogenic strain EGDe, shown as increased adhesion and invasion as well as enhanced cell-to-cell spread in cultured cell lines.
    Title: Activation of PrfA results in overexpression of virulence factors but does not rescue the pathogenicity of Listeria monocytogenes M7.
  9. These data indicated that loss of PrfA dramatically altered gene expression patterns in Listeria monocytogenes biofilm and resulted in reduced ability of the biofilm formation.
    Title: PrfA led to reduced biofilm formation and contributed to altered gene expression patterns in biofilm-forming Listeria monocytogenes.
  10. About 40% of Listeria monocytogenes isolates (three subtypes) were found to potentially present attenuated virulence because of the presence of mutations in the prfA and inlA genes
    Title: Low potential virulence associated with mutations in the inlA and prfA genes in Listeria monocytogenes isolated from raw retail poultry meat.
Submit: New GeneRIF Correction See all GeneRIFs (20)

General gene information

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Homology

regulatory

  • Loc: c2619440-2619435 regulatory_class = ribosome_binding_site

General protein information

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Names
peptide chain release factor 1
NP_466066.1
  • recognizes the termination signals UAG and UAA during protein translation a specificity which is dependent on amino acid residues residing in loops of the L-shaped tRNA-like molecule of RF1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003210.1 Reference assembly

    Range
    2619415..2620491 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_466066.1 peptide chain release factor 1 [Listeria monocytogenes EGD-e]

    See identical proteins and their annotated locations for NP_466066.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A608GHH4, A0A612SQJ4
    Conserved Domains (1) summary
    PRK00591
    Location:1 → 356
    prfA; peptide chain release factor 1; Validated
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8Y4A8.1

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