AJUBA ajuba LIM protein [Homo sapiens (human)] - Gene

Summary

Official Symbol: AJUBAprovided by HGNC
Official Full Name: ajuba LIM proteinprovided by HGNC
Primary source: HGNC:HGNC:20250
See related: Ensembl:ENSG00000129474 MIM:609066; AllianceGenome:HGNC:20250
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: JUB
Summary: Enables alpha-catenin binding activity and transcription corepressor activity. Involved in several processes, including negative regulation of hippo signaling; positive regulation of gene silencing by miRNA; and regulation of cellular response to hypoxia. Acts upstream of or within gene silencing by miRNA and positive regulation of protein-containing complex assembly. Located in several cellular components, including Golgi apparatus; P-body; and nucleoplasm. [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in skin (RPKM 17.2), liver (RPKM 8.4) and 15 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 14q11.2

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	14	NC_000014.9 (22971177..22982551, complement)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	14	NC_060938.1 (17171795..17183169, complement)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	14	NC_000014.8 (23440386..23451760, complement)

Chromosome 14 - NC_000014.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

LIM domain-containing protein Ajuba inhibits chemotherapy-induced apoptosis by negatively regulating p53 stability in colorectal cancer cells.
Title: LIM domain-containing protein Ajuba inhibits chemotherapy-induced apoptosis by negatively regulating p53 stability in colorectal cancer cells.
Ajuba Overexpression Promotes Breast Cancer Chemoresistance and Glucose Uptake through TAZ-GLUT3/Survivin Pathway.
Title: Ajuba Overexpression Promotes Breast Cancer Chemoresistance and Glucose Uptake through TAZ-GLUT3/Survivin Pathway.
miR-433-3p Targets AJUBA to Inhibit Malignant Progression of Glioma.
Title: miR-433-3p Targets AJUBA to Inhibit Malignant Progression of Glioma.
Ajuba transactivates N-cadherin expression in colorectal cancer cells through interaction with Twist.
Title: Ajuba transactivates N-cadherin expression in colorectal cancer cells through interaction with Twist.
Mitophagy antagonism by ZIKV reveals Ajuba as a regulator of PINK1 signaling, PKR-dependent inflammation, and viral invasion of tissues.
Title: Mitophagy antagonism by ZIKV reveals Ajuba as a regulator of PINK1 signaling, PKR-dependent inflammation, and viral invasion of tissues.
Hsa_circ_0128846 promotes tumorigenesis of colorectal cancer by sponging hsa-miR-1184 and releasing AJUBA and inactivating Hippo/YAP signalling.
Title: Hsa_circ_0128846 promotes tumorigenesis of colorectal cancer by sponging hsa-miR-1184 and releasing AJUBA and inactivating Hippo/YAP signalling.
Super-enhancer-driven AJUBA is activated by TCF4 and involved in epithelial-mesenchymal transition in the progression of Hepatocellular Carcinoma.
Title: Super-enhancer-driven AJUBA is activated by TCF4 and involved in epithelial-mesenchymal transition in the progression of Hepatocellular Carcinoma.
Knockout of Ajuba Attenuates the Growth and Migration of Hepatocellular Carcinoma Cells.
Title: Knockout of Ajuba Attenuates the Growth and Migration of Hepatocellular Carcinoma Cells.
Ajuba: An emerging signal transducer in oncogenesis.
Title: Ajuba: An emerging signal transducer in oncogenesis.
Ajuba functions as a co-activator of SP1 to induce its target gene, and that Ajuba itself is a target genes of SP1. Ajuba/SP1 complex could form a feed forward loop to drive SP1 target gene transcription and promote cell proliferation of pancreatic cancer cells.
Title: The LIM protein Ajuba/SP1 complex forms a feed forward loop to induce SP1 target genes and promote pancreatic cancer cell proliferation.

Submit: New GeneRIF Correction See all GeneRIFs (34)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genome-wide association study of primary tooth eruption identifies pleiotropic loci associated with height and craniofacial distances. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

WI-17421 (e-PCR)
- UniSTS:62850

RH47855 (e-PCR)
- UniSTS:65414

RH68535 (e-PCR)
- UniSTS:42171

G59876 (e-PCR)
- UniSTS:137159

MARC_11631-11632:1002817108:1 (e-PCR)
- UniSTS:267252

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables actin filament binding	IEA Inferred from Electronic Annotation more info
enables alpha-catenin binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activator activity	IEA Inferred from Electronic Annotation more info
enables transcription corepressor activity	IBA Inferred from Biological aspect of Ancestor more info
enables transcription corepressor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules	IEA Inferred from Electronic Annotation more info
involved_in cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in focal adhesion assembly	IEA Inferred from Electronic Annotation more info
involved_in glycerophospholipid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in lamellipodium assembly	IEA Inferred from Electronic Annotation more info
involved_in miRNA-mediated gene silencing by inhibition of translation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within miRNA-mediated post-transcriptional gene silencing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of hippo signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of hippo signaling	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of canonical NF-kappaB signal transduction	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cellular biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of protein-containing complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in protein localization	IEA Inferred from Electronic Annotation more info
involved_in regulation of DNA-templated transcription	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of cell migration	IEA Inferred from Electronic Annotation more info
involved_in regulation of cellular response to hypoxia	IDA Inferred from Direct Assay more info	PubMed
involved_in response to hypoxia	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to hypoxia	IDA Inferred from Direct Assay more info	PubMed
involved_in wound healing, spreading of epidermal cells	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IDA Inferred from Direct Assay more info
is_active_in P-body	IBA Inferred from Biological aspect of Ancestor more info
located_in P-body	IDA Inferred from Direct Assay more info	PubMed
is_active_in adherens junction	IBA Inferred from Biological aspect of Ancestor more info
located_in cell-cell junction	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info
located_in cytosol	TAS Traceable Author Statement more info
located_in focal adhesion	IEA Inferred from Electronic Annotation more info
located_in lamellipodium	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
part_of transcription regulator complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: LIM domain-containing protein ajuba

Names: jub, ajuba homolog

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001289097.2 → NP_001276026.1 LIM domain-containing protein ajuba isoform 3

See identical proteins and their annotated locations for NP_001276026.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks multiple 3' coding exons and contains an alternate 3' terminal exon, resulting in a different 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (3) has a distinct C-terminus and is shorter than isoform 1.

Source sequence(s)

AA909473, AL132780, BC034968, BX109281

UniProtKB/Swiss-Prot

Q96IF1
NM_032876.6 → NP_116265.1 LIM domain-containing protein ajuba isoform 1

See identical proteins and their annotated locations for NP_116265.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

Source sequence(s)

AL132780

Consensus CDS

CCDS9581.1

UniProtKB/Swiss-Prot

A8MX18, D3DS37, Q96IF1

Related

ENSP00000262713.2, ENST00000262713.7

Conserved Domains (4) summary

PHA03418
Location:18 → 103

PHA03418; hypothetical E4 protein; Provisional

cd09352
Location:338 → 391

LIM1_Ajuba_like; The first LIM domain of Ajuba-like proteins

cd09355
Location:403 → 455

LIM2_Ajuba_like; The second LIM domain of Ajuba-like proteins

cd09438
Location:463 → 524

LIM3_Ajuba_like; The third LIM domain of Ajuba-like proteins
NM_198086.3 → NP_932352.1 LIM domain-containing protein ajuba isoform 2

See identical proteins and their annotated locations for NP_932352.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1.

Source sequence(s)

AL132780

Consensus CDS

CCDS9582.1

UniProtKB/Swiss-Prot

Q96IF1

Related

ENSP00000380543.3, ENST00000397388.7

Conserved Domains (1) summary

cl02475
Location:46 → 107

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers